TY - JOUR
T1 - NeuroD1 Expression in Human Prostate Cancer
T2 - Can It Contribute to Neuroendocrine Differentiation Comprehension?
AU - Cindolo, Luca
AU - Franco, Renato
AU - Cantile, Monica
AU - Schiavo, Giulia
AU - Liguori, Giuseppina
AU - Chiodini, Paolo
AU - Salzano, Liugi
AU - Autorino, Riccardo
AU - Di Blasi, Arturo
AU - Falsaperla, Mario
AU - Feudale, Elisa
AU - Botti, Gerardo
AU - Gallo, Antonio
AU - Cillo, Clemente
PY - 2007/11
Y1 - 2007/11
N2 - Objectives: Neuroendocrine differentiation is a common feature of prostate cancer (pCA). NeuroD1 is a neuronal transcription factor able to convert epithelial cells into neurons. The aim of the study is to investigate NeuroD1 expression and compare it with chromogranin-A, synaptophysin, and CD56 staining in human prostate cell lines and surgical specimens. Methods: We detected NeuroD1 gene expression, by duplex reverse transcriptase-polymerase chain reaction, in primary human prostate fibroblasts, in EPN, LNCaP, DU145, and PC3 cell lines before and after cAMP exposure, in 6 BPH and 11 pCA samples. Thereafter 166 paraffin sections from normal and neoplastic prostates were stained with NeuroD1, chromogranin-A, synaptophysin, and CD56 antibodies. The relationships between chromogranin-A and NeuroD1 and clinicopathologic parameters were evaluated by multivariate logistic regression analysis. Results: NeuroD1 is inactive in baseline prostate cell lines and BPHs, whereas it is actively expressed in cAMP-treated EPN, PC3, and DU145 cells. In our surgical series, positive chromogranin-A, synaptophysin, CD56, and NeuroD1 staining was detected in 26.5%, 4.3%, 3.1%, and 35.5%, respectively (difference between chromogranin-A and NeuroD1: p <0.05). The multivariate analysis showed a strong association between chromogranin-A and microscopic perineural invasion (OR: 2.49; 95%CI, 0.85-7.32; p = 0.097) and a high primary Gleason score (OR: 1.96; 95%CI, 1.14-3.39; p = 0.015), whereas NeuroD1 expression strictly correlated to microscopic perineural invasion (OR: 2.97; 95%CI, 1.05-8.41; p = 0.04). Conclusions: Expression of NeuroD1 versus chromogranin-A is more frequent in pCA, and correlates to increased indicators of malignancy in moderately to poorly differentiated pCA, and could be involved in the pathophysiology of the neuroendocrine differentiation of pCA.
AB - Objectives: Neuroendocrine differentiation is a common feature of prostate cancer (pCA). NeuroD1 is a neuronal transcription factor able to convert epithelial cells into neurons. The aim of the study is to investigate NeuroD1 expression and compare it with chromogranin-A, synaptophysin, and CD56 staining in human prostate cell lines and surgical specimens. Methods: We detected NeuroD1 gene expression, by duplex reverse transcriptase-polymerase chain reaction, in primary human prostate fibroblasts, in EPN, LNCaP, DU145, and PC3 cell lines before and after cAMP exposure, in 6 BPH and 11 pCA samples. Thereafter 166 paraffin sections from normal and neoplastic prostates were stained with NeuroD1, chromogranin-A, synaptophysin, and CD56 antibodies. The relationships between chromogranin-A and NeuroD1 and clinicopathologic parameters were evaluated by multivariate logistic regression analysis. Results: NeuroD1 is inactive in baseline prostate cell lines and BPHs, whereas it is actively expressed in cAMP-treated EPN, PC3, and DU145 cells. In our surgical series, positive chromogranin-A, synaptophysin, CD56, and NeuroD1 staining was detected in 26.5%, 4.3%, 3.1%, and 35.5%, respectively (difference between chromogranin-A and NeuroD1: p <0.05). The multivariate analysis showed a strong association between chromogranin-A and microscopic perineural invasion (OR: 2.49; 95%CI, 0.85-7.32; p = 0.097) and a high primary Gleason score (OR: 1.96; 95%CI, 1.14-3.39; p = 0.015), whereas NeuroD1 expression strictly correlated to microscopic perineural invasion (OR: 2.97; 95%CI, 1.05-8.41; p = 0.04). Conclusions: Expression of NeuroD1 versus chromogranin-A is more frequent in pCA, and correlates to increased indicators of malignancy in moderately to poorly differentiated pCA, and could be involved in the pathophysiology of the neuroendocrine differentiation of pCA.
KW - CD56
KW - Chromogranin A
KW - NeuroD1
KW - Neuroendocrine differentiation
KW - Prostate cancer
KW - Synaptophysin
UR - http://www.scopus.com/inward/record.url?scp=34648844152&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34648844152&partnerID=8YFLogxK
U2 - 10.1016/j.eururo.2006.11.030
DO - 10.1016/j.eururo.2006.11.030
M3 - Article
C2 - 17126478
AN - SCOPUS:34648844152
VL - 52
SP - 1365
EP - 1373
JO - European Urology
JF - European Urology
SN - 0302-2838
IS - 5
ER -