Neurogenesis in temporal lobe epilepsy: Relationship between histological findings and changes in dentate gyrus proliferative properties

Gianluca Marucci, Marco Giulioni, Guido Rubboli, Michela Paradisi, Mercedes Fernández, Giovanna Del Vecchio, Eugenio Pozzati

Research output: Contribution to journalArticle

Abstract

Objective: The relationship between hippocampal histopathological abnormalities, epileptogenesis and neurogenesis remains rather unclear. Methods: Tissue samples including the subgranular zone of dentate gyrus (DG) were freshly collected for tissue culture for neurospheres generation in 16 patients who underwent surgery for drug-resistant temporal lobe epilepsy. Remaining tissues were histologically examined to assess the presence of mesial temporal sclerosis (MTS) and focal cortical dysplasia. Results: MTS was detected in 8 cases. Neurospheres were formed in 10/16 cases. Only three out of these 10 cases exhibited MTS; on the contrary 5/6 cases lacking neurosphere proliferation presented MTS. There was a significant correlation between presence of MTS and absence of proliferation (p = 0.0389). We also observed a correlation between history of febrile seizures (FS) and presence of MTS (p = 0.0004) and among the 6 cases lacking neurosphere proliferation, 4 cases (66.6%) had experienced prolonged FS. Among "proliferating" cases the percentage of granular cells pathology (GCP) was lower (20% vs 50%) compared to "non proliferating" cases. Conclusion: A decreased potential to generate neurosphere from the SGZ is related to MTS and to alterations of dentate gyrus granule cells, especially in MTS type 1b and GCP type 1. These histological findings may have different prognostic implications, regarding seizure and neuropsychological outcome, compared to patients with other epileptogenic lesions (such as FCD, glioneuronal tumours, vascular lesions).

Original languageEnglish
Pages (from-to)187-191
Number of pages5
JournalClinical Neurology and Neurosurgery
Volume115
Issue number2
DOIs
Publication statusPublished - Feb 2013

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Keywords

  • Dentate gyrus
  • Epilepsy surgery
  • Epileptogenesis
  • Febrile seizures
  • Neurogenesis
  • Temporal lobe epilepsy

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

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