Neuroglobin modification by reactive quinone species

Stefania Nicolis, Enrico Monzani, Alessandro Pezzella, Paolo Ascenzi, Diego Sbardella, Luigi Casella

Research output: Contribution to journalArticle

Abstract

The physiological functions of neuroglobin (Ngb), the heme protein of the globin family expressed in the nervous tissue, have not yet been clarified. Besides O2 storage and homeostasis, Ngb is thought to play a role in neuroprotection as a scavenger of toxic reactive species generated in vivo under conditions of oxidative stress. Herein, the interaction of Ngb with the quinones generated by oxidation of catecholamines (dopamine, norepinephrine) and catechol estrogens (2-hydroxyestradiol and 4-hydroxyestradiol), which have been implicated in neurodegenerative pathologies like Parkinson's and Alzheimer's diseases, has been investigated. The cytotoxicity of quinones has been ascribed to the derivatization of amino acid residues (mainly cysteine) in proteins through the formation of covalent bonds with the aromatic rings. Combined studies of tandem mass spectrometry and protein unfolding indicate the presence of quinone-promoted modifications in all of the Ngb derivatives analyzed (i.e., obtained employing either catecholamines or catechol estrogens as the source of the reactive species). Among protein residues, the highest reactivity of cysteines (Cys46, Cys55, and Cys120 in human Ngb) toward quinone species has been confirmed, and the dependence of the extent of protein modification on the method employed for catechol oxidation has been observed. When the oxidation reaction proceeds by one-electron steps, the involvement of semiquinone reactivity has been observed. The whole analysis of the data of Ngb modification suggests that the catecholamine-oxidation products can extensively modify proteins (likely by catecholamine oligomers, the compounds initially formed during the transformation of catecholamine to melanin). The modification mediated by catechol estrogens is less pronounced but strongly affects the interactions with the solvent as well as the protein stability.

Original languageEnglish
Pages (from-to)1821-1831
Number of pages11
JournalChemical Research in Toxicology
Volume26
Issue number12
DOIs
Publication statusPublished - Dec 16 2013

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

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    Nicolis, S., Monzani, E., Pezzella, A., Ascenzi, P., Sbardella, D., & Casella, L. (2013). Neuroglobin modification by reactive quinone species. Chemical Research in Toxicology, 26(12), 1821-1831. https://doi.org/10.1021/tx4001896