Neuroimmune interactions at different intestinal sites are related to abdominal pain symptoms in children with IBS

G. Di Nardo, G. Barbara, S. Cucchiara, C. Cremon, R. J. Shulman, S. Isoldi, L. Zecchi, L. Drago, S. Oliva, R. Saulle, M. R. Barbaro, L. Stronati

Research output: Contribution to journalArticle

Abstract

Background: Neuroimmune interactions and inflammation have been proposed as factors involved in sensory-motor dysfunction and symptom generation in adult irritable bowel syndrome (IBS) patients. In children with IBS and healthy controls, we measured ileocolonic mast cell infiltration and fecal calprotectin and evaluated the relationships between these parameters and abdominal pain symptoms and stooling pattern. Methods: Irritable bowel syndrome patients diagnosed according to Pediatric Rome III criteria and healthy controls kept a 2-week pain/stooling diary. Ileocolonic mucosal mast cells (MC) and MC in close proximity to nerve fibers (MC-NF) were identified immunohistochemically and quantified. Fecal calprotectin concentration was measured. Key Results: 21 IBS patients and 10 controls were enrolled. The MC-NF count was significantly higher in the ileum (p = 0.01), right colon (p = 0.04), and left colon (p <0.001) of IBS patients compared with controls. No differences in fecal calprotectin concentration were noted. Abdominal pain intensity score correlated with ileal MC count (rs = 0.47, p = 0.030) and right colon MC-NF count (rs = 0.52, p = 0.015). In addition, children with IBS with >3 abdominal pain episodes/week had greater ileal (p = 0.002) and right colonic (p = 0.01) MC counts and greater ileal (p = 0.05) and right colonic (p = 0.016) MC-NF counts than children with less frequent pain. No relationship was found between MC and MC-NF and fecal calprotectin or stooling pattern. Conclusions & Inferences: Mast cells-nerve fibers counts are increased in the ileocolonic mucosa of children with IBS. Mast cells and MC-NF counts are related to the intensity and frequency of abdominal pain. The relevance of mast cells and neuroimmune interaction in children with IBS is still unknown. We assessed in the ileal and colonic mucosa of children with IBS mast cell infiltration, mast cell-nerve interactions, and their correlations with symptoms. The number of mast cells in close proximity to nerves both in the ileal and colonic mucosa was increased and related to the intensity and frequency of abdominal pain.

Original languageEnglish
Pages (from-to)196-204
Number of pages9
JournalNeurogastroenterology and Motility
Volume26
Issue number2
DOIs
Publication statusPublished - Feb 2014

Keywords

  • Children
  • Functional gastrointestinal disorders
  • Inflammation
  • Irritable bowel syndrome

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

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    Di Nardo, G., Barbara, G., Cucchiara, S., Cremon, C., Shulman, R. J., Isoldi, S., Zecchi, L., Drago, L., Oliva, S., Saulle, R., Barbaro, M. R., & Stronati, L. (2014). Neuroimmune interactions at different intestinal sites are related to abdominal pain symptoms in children with IBS. Neurogastroenterology and Motility, 26(2), 196-204. https://doi.org/10.1111/nmo.12250