Neuroligin 1 induces blood vessel maturation by cooperating with the α6 integrin

Anna Valeria Samarelli, Elena Riccitelli, Laura Bizzozero, Tatiana Nunes Silveira, Giorgio Seano, Margherita Pergolizzi, Grazia Vitagliano, Ilaria Cascone, Gilles Carpentier, Alessia Bottos, Luca Primo, Federico Bussolino, Marco Arese

Research output: Contribution to journalArticlepeer-review


The synaptic protein Neuroligin 1 (NLGN1), a cell adhesion molecule, is critical for the formation and consolidation of synaptic connectivity and is involved in vascular development. The mechanism through which NLGN1 acts, especially in vascular cells, is unknown. Here, we aimed at deepening our knowledge on the cellular activities and molecular pathways exploited by endothelial NLGN1 both in vitro and in vivo. We analyzed the phenotypic consequences of NLGN1 expression modulation in endothelial cells through in vitro angiogenesis assays and the mouse postnatal retinal angiogenesis model. We demonstrate that NLGN1, whereas not affecting endothelial cell proliferation or migration, modulates cell adhesion to the vessel stabilizing protein laminin through cooperation with the α6 integrin, a specific laminin receptor. Finally, we show that in vivo, NLGN1 and α6 integrin preferentially colocalize in the mature retinal vessels, whereas NLGN1 deletion causes an aberrant VE-cadherin, laminin and α6 integrin distribution in vessels, along with significant structural defects in the vascular tree.

Original languageEnglish
Pages (from-to)19466-19476
Number of pages11
JournalJournal of Biological Chemistry
Issue number28
Publication statusPublished - Jul 11 2014

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology


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