Neurological disorders are often found in AIDS patients. HIV, as well as other opportunistic pathogens such as Cytomegalovirus (CMV), can cause neurological disorders. Diagnosis of CMV infection in the central nervous system (CNS) is infrequent because of the low sensibility and specificity of standard methods (cell cultures, serology). We set up a rapid and reliable n- PCR method for the detection of CMV-DNA in the cerebrospinal fluid (CSF) of patients affected by AIDS with suspect CMV infection of the CNS. Forty-one consecutive CSF samples were tested. Eight were positive by n-PCR (19.5%). The most frequent clinical manifestation in these patients was encephalitis. These preliminary results suggest that molecular biology techniques such as n-PCR can be used for the diagnosis of CMV infection in the CNS, especially when symptomatology, CSF tests and image diagnosis do not offer significant sensibility.
|Translated title of the contribution||Neurological disorders in AIDS patients: Cytomegalovirus-DNA detection by nested polymerase chain reaction in cerebrospinal fluid|
|Number of pages||3|
|Publication status||Published - 1997|
ASJC Scopus subject areas
- Microbiology (medical)