Neuronal plasticity in aging: A quantitative immunohistochemical study of GAP-43 distribution in discrete regions of the rat brain

Tiziana Casoli, Carla Spagna, Patrizia Fattoretti, Rosaria Gesuita, Carlo Bertoni-Freddari

Research output: Contribution to journalArticle

Abstract

Age-related changes in neuroplasticity have been investigated considering the neuronal growth-associated protein GAP-43 as a marker of nerve cell structural adaptive capabilities. We carried out a quantitative immunohistochemical study on the distribution of GAP-43 in the molecular layer of the cerebellar cortex, in the inner molecular layer of the hippocampal dentate gyrus, in the stratum radiatum of the CA1 region, in layer 1 of the cingulate cortex and in the nerve fiber layer of the main olfactory bulb of 3-, 18- and 31-month-old Wistar rats. A decrease of GAP-43 immunoreactivity was observed in the old rats in comparison with the adult animals in all the 5 areas analyzed, although these variations were only statistically significant in the dentate gyrus, cingulate cortex and olfactory bulb. In these latter zones, GAP-43 immunolabeling is reduced by 54, 42 and 38%, respectively, in the old versus the adult group. Comparing these data with the age-dependent decrease of neuron density innervating the areas investigated, we support the hypothesis that the decline of GAP-43 observed in old animals documents a consistent reduction of axon plasticity in the inner molecular layer of the dentate gyrus and in layer 1 of the cingulate cortex. These results suggest an important role of GAP-43 as a marker of age-dependent deterioration of synaptic plasticity, especially in those areas of the brain involved in memory and emotional behavior.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalBrain Research
Volume714
Issue number1-2
DOIs
Publication statusPublished - Apr 1 1996

Keywords

  • Aging
  • Cerebellum
  • Cingulate cortex
  • GAP-43
  • Hippocampus
  • Neuronal plasticity
  • Olfactory bulb
  • Quantitative immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

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