Neuropathy and anti-myelin-associated glycoprotein IgM M proteins

T cell regulation of M protein secretion in vitro

N. Latov, M. Godfrey, Y. Thomas, E. Nobile-Orazio, L. Spatz, J. Abraham, G. Perman, L. Freddo, L. Chess

Research output: Contribution to journalArticle

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Abstract

In patients with plasma cell dyscrasia, individual clones of antibody-producing cells proliferate abnormally and secrete monoclonal antibodies or M proteins in excess. The cause of the monoclonal proliferation of lymphocytes and M protein secretion is unknown and it is not known whether the M protein-secreting B cells are autonomous or capable of responding to regulatory T cells. We carried out experiments using lymphocytes from a patient with neuropathy and plasma cell dyscrasia whose IgM M protein bound to the myelin-associated glycoporotein (MAG) to determine whether secretion of the M protein in vitro was responsive to T cell help or suppression. M protein secretion was measured by an enzyme-linked immunosorbent assay system for measuring anti-MAG IgM, and the number of M protein-secreting lymphocytes was enumerated by a reverse hemolytic plaque assay specific for the M protein idiotype. The patient's B cells were maximally stimulated by pokeweed mitogen-activated autologous OKT4 + T-helper cells and the helper effect was inhibited by OKT8 + suppressor/cytotoxic T cells. Low levels of M protein secretion in the absence of T cells were also observed and there was partial stimulation of M protein secretion by T cells in the absence of pokeweed mitogen.

Original languageEnglish
Pages (from-to)182-188
Number of pages7
JournalAnnals of Neurology
Volume18
Issue number2
DOIs
Publication statusPublished - 1985

Fingerprint

Myelin-Associated Glycoprotein
Immunoglobulin M
T-Lymphocytes
Proteins
Pokeweed Mitogens
Paraproteinemias
Lymphocytes
Myelin Sheath
B-Lymphocytes
In Vitro Techniques
Antibody-Producing Cells
Regulatory T-Lymphocytes
Helper-Inducer T-Lymphocytes
Clone Cells
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuropathy and anti-myelin-associated glycoprotein IgM M proteins : T cell regulation of M protein secretion in vitro. / Latov, N.; Godfrey, M.; Thomas, Y.; Nobile-Orazio, E.; Spatz, L.; Abraham, J.; Perman, G.; Freddo, L.; Chess, L.

In: Annals of Neurology, Vol. 18, No. 2, 1985, p. 182-188.

Research output: Contribution to journalArticle

Latov, N, Godfrey, M, Thomas, Y, Nobile-Orazio, E, Spatz, L, Abraham, J, Perman, G, Freddo, L & Chess, L 1985, 'Neuropathy and anti-myelin-associated glycoprotein IgM M proteins: T cell regulation of M protein secretion in vitro', Annals of Neurology, vol. 18, no. 2, pp. 182-188. https://doi.org/10.1002/ana.410180204
Latov, N. ; Godfrey, M. ; Thomas, Y. ; Nobile-Orazio, E. ; Spatz, L. ; Abraham, J. ; Perman, G. ; Freddo, L. ; Chess, L. / Neuropathy and anti-myelin-associated glycoprotein IgM M proteins : T cell regulation of M protein secretion in vitro. In: Annals of Neurology. 1985 ; Vol. 18, No. 2. pp. 182-188.
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