Neuropilin-1 is upregulated in Sjögren's syndrome and contributes to pathological neovascularization

Margherita Sisto, Sabrina Lisi, Dario Domenico Lofrumento, Massimo D'Amore, Domenico Ribatti

Research output: Contribution to journalArticlepeer-review

Abstract

Neuropilin-1 (NRP1) is a transmembrane co-receptor for members of the vascular endothelial growth factor family. Recent studies revealed an important role of NRP1 in angiogenesis and progression of many diseases. The role of NRP1 in the development of Sjögren's syndrome (SS), one of the most common rheumatic diseases, has not yet been investigated. Molecular studies and protein expression techniques were performed to elucidate the gene and protein expression proWle of NRP1 in human salivary gland epithelial cells (SGEC) from primary SS. We used human microarrays and transient transfection with a mutant form of the negative inhibitory kBα proteins (I kBαDN) to investigate whether selective inhibition of nuclear Factor-kB (NF-kB) improves NRP1-mediated pro-angiogenic factors release from SS SGEC. The selective NRP1 function inhibition with an antibody to human NRP1, was employed to evaluate the therapeutic potential of targeting NRP1. We demonstrate that NRP1 is expressed in SGEC of both human healthy biopsies and in SS samples, and increased NRP1 expression in SS SGEC is signiWcantly associated with pro-angiogenic factors release. Neutralizing anti- NRP1 antibody decreased pro-angiogenic factor production from SS SGEC and blocking NF-kB activation could be a way to inhibit NRP1-mediated angiogenesis in Sjögren's syndrome.

Original languageEnglish
Pages (from-to)669-677
Number of pages9
JournalHistochemistry and Cell Biology
Volume137
Issue number5
DOIs
Publication statusPublished - May 2012

Keywords

  • I BαSalivary gland
  • Neuropilin
  • NFB
  • Sjögren's syndrome

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Medical Laboratory Technology
  • Molecular Biology

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