We have examined the effect of various chemokines on neuronal toxicity in culture. In mixed cortical cultures, challenged with a brief pulse of N-methyl-D-aspartate (NMDA, 60 μM, 10 min), chemokines were either present for 2 h preceding the pulse or they were co-applied with NMDA and then kept in the medium for the following 20-24 h. Interleukin-8 (IL-8), regulated on activation of normal T cells expressed and secreted (RANTES) and macrophage/monocyte chemoattractant protein-1 (MCP-1), were neuroprotective under both conditions, whereas stromal cell-derived factor 1α (SDF-1α) was protective only when applied during and after the NMDA pulse. Mixed or pure neuronal cultures were also exposed for 48 h to a toxic fragment of the β-amyloid peptide (β-amyloid peptide-(25-35), 12.5 or 25 μM) in the absence or presence of chemokines. Among a number of chemokines, only RANTES was neuroprotective against β-amyloid peptide-(25-35)-induced neurotoxicity in both cultures. We conclude that activation of chemokine receptors differentially affects neuronal degeneration induced by excitotoxins or β-amyloid peptide in cortical cultures. Copyright (C) 2000 Elsevier Science B.V.
- Cortical culture
- N-methyl-D-aspartate (NMDA)
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience