Neuroprotective coordination of cell mitophagy by the ATPase Inhibitory Factor 1

Ivana Matic, Stefania Cocco, Caterina Ferraina, Rebeca Martin-Jimenez, F. Florenzano, James Crosby, Ramona Lupi, Giusy Amadoro, Claire Russell, Giuseppe Pignataro, Lucio Annunziato, Andrey Y. Abramov, Michelangelo Campanella

Research output: Contribution to journalArticle

Abstract

The mitochondrial ATPase Inhibitory Factor 1 (hereafter referred to as IF1) blocks the reversal of the F1Fo-ATPsynthase to prevent detrimental consumption of cellular ATP and associated demise. Herein, we infer further its molecular physiology by assessing its protective function in neurons during conditions of challenged homeostatic respiration. By adopting in vitro and in vivo protocols of hypoxia/ischemia and re-oxygenation, we show that a shift in the IF1:F1Fo-ATPsynthase expression ratio occurs in neurons. This increased IF1 level is essential to induce accumulation of the PTEN-induced putative kinase 1 (PINK-1) and recruitment of the mitophagic ubiquitin ligase PARK-2 to promote autophagic "control" of the mitochondrial population. In IF1 overexpressing neurons ATP depletion is reduced during hypoxia/ischemia and the mitochondrial membrane potential (ΔYm) resilient to re-oxygenation as well as resistant to electrogenic, Ca2+ dependent depolarization. These data suggest that in mammalian neurons mitochondria adapt to respiratory stress by upregulating IF1, which exerts a protective role by coordinating pro-survival cell mitophagy and bioenergetics resilience.

Original languageEnglish
Pages (from-to)56-68
Number of pages13
JournalPharmacological Research
Volume103
DOIs
Publication statusPublished - Jan 1 2016

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Mitochondrial Degradation
Adenosine Triphosphatases
Neurons
Ischemia
Adenosine Triphosphate
Mitochondrial Membrane Potential
Ligases
Ubiquitin
Energy Metabolism
Cell Survival
Mitochondria
Respiration
Population

Keywords

  • FF-ATPsynthase
  • Hypoxia/ischemia
  • IF
  • Mitophagy
  • Re-oxygenation
  • ΔΨ

ASJC Scopus subject areas

  • Pharmacology

Cite this

Neuroprotective coordination of cell mitophagy by the ATPase Inhibitory Factor 1. / Matic, Ivana; Cocco, Stefania; Ferraina, Caterina; Martin-Jimenez, Rebeca; Florenzano, F.; Crosby, James; Lupi, Ramona; Amadoro, Giusy; Russell, Claire; Pignataro, Giuseppe; Annunziato, Lucio; Abramov, Andrey Y.; Campanella, Michelangelo.

In: Pharmacological Research, Vol. 103, 01.01.2016, p. 56-68.

Research output: Contribution to journalArticle

Matic, I, Cocco, S, Ferraina, C, Martin-Jimenez, R, Florenzano, F, Crosby, J, Lupi, R, Amadoro, G, Russell, C, Pignataro, G, Annunziato, L, Abramov, AY & Campanella, M 2016, 'Neuroprotective coordination of cell mitophagy by the ATPase Inhibitory Factor 1', Pharmacological Research, vol. 103, pp. 56-68. https://doi.org/10.1016/j.phrs.2015.10.010
Matic I, Cocco S, Ferraina C, Martin-Jimenez R, Florenzano F, Crosby J et al. Neuroprotective coordination of cell mitophagy by the ATPase Inhibitory Factor 1. Pharmacological Research. 2016 Jan 1;103:56-68. https://doi.org/10.1016/j.phrs.2015.10.010
Matic, Ivana ; Cocco, Stefania ; Ferraina, Caterina ; Martin-Jimenez, Rebeca ; Florenzano, F. ; Crosby, James ; Lupi, Ramona ; Amadoro, Giusy ; Russell, Claire ; Pignataro, Giuseppe ; Annunziato, Lucio ; Abramov, Andrey Y. ; Campanella, Michelangelo. / Neuroprotective coordination of cell mitophagy by the ATPase Inhibitory Factor 1. In: Pharmacological Research. 2016 ; Vol. 103. pp. 56-68.
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