Neuroprotective effects of dihydroprogesterone and progesterone in an experimental model of nerve crush injury

I. Roglio, R. Bianchi, S. Gotti, S. Scurati, S. Giatti, M. Pesaresi, D. Caruso, G. C. Panzica, R. C. Melcangi

Research output: Contribution to journalArticle

Abstract

A satisfactory management to ensure a full restoration of peripheral nerve after trauma is not yet available. Using an experimental protocol, in which crush injury was applied 1 cm above the bifurcation of the rat sciatic nerve for 20 s, we here demonstrate that the levels of neuroactive steroids, such as pregnenolone and progesterone (P) metabolites (i.e. dihydroprogesterone, DHP, and tetrahydroprogesterone, THP) present in injured sciatic nerve were significantly decreased. On this basis, we have focused our attention on DHP and its direct precursor, P, analyzing whether these two neuroactive steroids may have neuroprotective effects on biochemical, functional and morphological alterations occurring during crush-induced degeneration-regeneration. We demonstrate that DHP and/or P counteract biochemical alterations (i.e. myelin proteins and Na+,K+-ATPase pump) and stimulate reelin gene expression. These two neuroactive steroids also counteract nociception impairment, and DHP treatment significantly decreases the up-regulation of myelinated fibers' density occurring in crushed animals. Altogether, these observations suggest that DHP and P (i.e. two neuroactive steroids interacting with progesterone receptor) may be considered protective agents in case of nerve crush injury.

Original languageEnglish
Pages (from-to)673-685
Number of pages13
JournalNeuroscience
Volume155
Issue number3
DOIs
Publication statusPublished - Aug 26 2008

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20-alpha-Dihydroprogesterone
Nerve Crush
Neuroprotective Agents
Progesterone
Theoretical Models
Steroids
Sciatic Nerve
Myelin Proteins
Protective Agents
Pregnenolone
Nociception
Progesterone Receptors
Peripheral Nerves
Regeneration
Up-Regulation
Gene Expression
Crush Injuries
Wounds and Injuries

Keywords

  • morphology
  • myelin proteins
  • neuroactive steroids
  • nociception
  • rat sciatic nerve
  • reelin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Roglio, I., Bianchi, R., Gotti, S., Scurati, S., Giatti, S., Pesaresi, M., ... Melcangi, R. C. (2008). Neuroprotective effects of dihydroprogesterone and progesterone in an experimental model of nerve crush injury. Neuroscience, 155(3), 673-685. https://doi.org/10.1016/j.neuroscience.2008.06.034

Neuroprotective effects of dihydroprogesterone and progesterone in an experimental model of nerve crush injury. / Roglio, I.; Bianchi, R.; Gotti, S.; Scurati, S.; Giatti, S.; Pesaresi, M.; Caruso, D.; Panzica, G. C.; Melcangi, R. C.

In: Neuroscience, Vol. 155, No. 3, 26.08.2008, p. 673-685.

Research output: Contribution to journalArticle

Roglio, I, Bianchi, R, Gotti, S, Scurati, S, Giatti, S, Pesaresi, M, Caruso, D, Panzica, GC & Melcangi, RC 2008, 'Neuroprotective effects of dihydroprogesterone and progesterone in an experimental model of nerve crush injury', Neuroscience, vol. 155, no. 3, pp. 673-685. https://doi.org/10.1016/j.neuroscience.2008.06.034
Roglio, I. ; Bianchi, R. ; Gotti, S. ; Scurati, S. ; Giatti, S. ; Pesaresi, M. ; Caruso, D. ; Panzica, G. C. ; Melcangi, R. C. / Neuroprotective effects of dihydroprogesterone and progesterone in an experimental model of nerve crush injury. In: Neuroscience. 2008 ; Vol. 155, No. 3. pp. 673-685.
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