Abstract
Neurosecretion competence is intended as the ability of neurosecretory cells to express dense and clear vesicles discharged by regulated exocytosis (neurotransmitter release). Such a property, which so far has never been studied independently, is investigated here by a heterotypic cell fusion approach, using a clone of rat pheochromocytoma PC12 cells totally incompetent for neurosecretion that still largely maintains its typical molecular and cellular phenotype. When fused with wild-type partners of various species (rat, human) and specialization (PC12, neuroblastoma SH- SY5Y, HeLa), the defective cells reacquire their competence as revealed by the expression of their secretion-specific proteins. Fused wild-type cells therefore appear able to complement defective cells by providing them with factor(s) inducing the reactivation of their secretory program. The mechanism of action of these factors may consist not in a coordinate unblocking of transcription but in the prevention of a rapid post-transcriptional degradation of the mRNAs for secretion-specific genes.
| Original language | English |
|---|---|
| Pages (from-to) | 34683-34686 |
| Number of pages | 4 |
| Journal | Journal of Biological Chemistry |
| Volume | 273 |
| Issue number | 52 |
| DOIs | |
| Publication status | Published - Dec 25 1998 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Neurosecretion competence, an independently regulated trait of the neurosecretory cell phenotype. / Borgonovo, Barbara; Racchetti, Gabriella; Malosio, Marialuisa; Benfante, Roberta; Podini, Paola; Rosa, Patrizia; Meldolesi, Jacopo.
In: Journal of Biological Chemistry, Vol. 273, No. 52, 25.12.1998, p. 34683-34686.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neurosecretion competence, an independently regulated trait of the neurosecretory cell phenotype
AU - Borgonovo, Barbara
AU - Racchetti, Gabriella
AU - Malosio, Marialuisa
AU - Benfante, Roberta
AU - Podini, Paola
AU - Rosa, Patrizia
AU - Meldolesi, Jacopo
PY - 1998/12/25
Y1 - 1998/12/25
N2 - Neurosecretion competence is intended as the ability of neurosecretory cells to express dense and clear vesicles discharged by regulated exocytosis (neurotransmitter release). Such a property, which so far has never been studied independently, is investigated here by a heterotypic cell fusion approach, using a clone of rat pheochromocytoma PC12 cells totally incompetent for neurosecretion that still largely maintains its typical molecular and cellular phenotype. When fused with wild-type partners of various species (rat, human) and specialization (PC12, neuroblastoma SH- SY5Y, HeLa), the defective cells reacquire their competence as revealed by the expression of their secretion-specific proteins. Fused wild-type cells therefore appear able to complement defective cells by providing them with factor(s) inducing the reactivation of their secretory program. The mechanism of action of these factors may consist not in a coordinate unblocking of transcription but in the prevention of a rapid post-transcriptional degradation of the mRNAs for secretion-specific genes.
AB - Neurosecretion competence is intended as the ability of neurosecretory cells to express dense and clear vesicles discharged by regulated exocytosis (neurotransmitter release). Such a property, which so far has never been studied independently, is investigated here by a heterotypic cell fusion approach, using a clone of rat pheochromocytoma PC12 cells totally incompetent for neurosecretion that still largely maintains its typical molecular and cellular phenotype. When fused with wild-type partners of various species (rat, human) and specialization (PC12, neuroblastoma SH- SY5Y, HeLa), the defective cells reacquire their competence as revealed by the expression of their secretion-specific proteins. Fused wild-type cells therefore appear able to complement defective cells by providing them with factor(s) inducing the reactivation of their secretory program. The mechanism of action of these factors may consist not in a coordinate unblocking of transcription but in the prevention of a rapid post-transcriptional degradation of the mRNAs for secretion-specific genes.
UR - http://www.scopus.com/inward/record.url?scp=0032567526&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032567526&partnerID=8YFLogxK
U2 - 10.1074/jbc.273.52.34683
DO - 10.1074/jbc.273.52.34683
M3 - Article
C2 - 9856988
AN - SCOPUS:0032567526
VL - 273
SP - 34683
EP - 34686
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 52
ER -