Neurotensin-amplification of DNA synthesis stimulated by EGF or TGFα in primary cultures of adult rat hepatocytes

K. Hasegawa, B. I. Carr

Research output: Contribution to journalArticlepeer-review

Abstract

Epidermal growth factor and transforming growth factor alpha stimulated DNA synthesis in primary cultures of adult rat hepatocytes. In the presence of neurotensin, the stimulatory effect of epidermal growth factor or transforming growth factor α on DNA synthesis was enhanced 3- to 5-fold. Since neurotensin by itself did not stimulate DNA synthesis at all concentrations tested, enhancement of DNA synthesis by neurotensin is due to the amplification of the effect of epidermal growth factor or transforming growth factor α. The amplification by neurotensin was observed in a dose-dependent manner with a maximal effect at 10-8 M, although its effect was significantly observed at as low as 10-10 M. This neurotensin amplification of DNA synthesis was observed when hepatocytes were cultured in Williams' medium E, but not in Leibovitz L-15 medium, suggesting that minor components in the medium are required for hepatocytes to fully respond to neurotensin. Neurotensin-related peptides such as kinetensin or neuromedin-N did not have this amplifying effect on DNA synthesis at any concentration tested. From these results, neurotensin can be regarded as a new secondary mitogen

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalCell Structure and Function
Volume18
Issue number2
Publication statusPublished - 1993

Keywords

  • Hepatocytes
  • Mitogen
  • Neurotensin
  • Peptide

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Structural Biology

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