Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis

Alberto Ricci, Salvatore Mariotta, Cesare Saltini, Carlo Falasca, Maria Rosaria Giovagnoli, Francesco Mannino, Paolo Graziano, Salvatore Sciacchitano, Francesco Amenta

Research output: Contribution to journalArticle

Abstract

Background and aim of the study: Pulmonary sarcoidosis is a chronic granulomatous disease characterized by macrophage and CD4+ T-cell accumulation at the site of inflammation. Analysis of the cytokine network has substantially improved knowledge on immunopathogenesis of sarcoidosis. We hypothesize that neurotrophins (NTs), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and NT-3, besides their importance in immune system activities, participate in chronic inflammatory disorders and in repair processes. Methods: The expression of NTs and NT receptors was assessed in broncho alveolar lavage (BAL) macrophages, CD4 and CD8 T-cells, from 10 patients with pulmonary sarcoidosis, using molecular biology, Western blotting and immunocytochemistry. Results: Increased levels of NTs and of high affinity NT receptor (Trks) transcripts and proteins in BAL macrophages, CD4+ and CD8+ T-cells from pulmonary sarcoidosis patients were demonstrated in comparison with healthy controls. Contrarily to healthy controls, in pulmonary sarcoidosis the expression of NGF was increased in alveolar macrophages as well as NGF and BDNF in CD4+ and CD8+ T-cells. An increased expression of TrkA, TrkB and TrkC receptors was also noticeable. Furthermore, BDNF expression in alveolar macrophages and NT-3 expression in the three different BAL immune cell populations investigated were induced during sarcoidosis. A significant correlation was observed between CD4:CD8 ratio, lymphocytosis, radiological stage and CD4 and CD8 NT expression. Conclusions: These findings suggest that NTs are exaggeratedly expressed in BAL immune cells in pulmonary sarcoidosis and may participate in the progression of disease modulating immune cell functions.

Original languageEnglish
Pages (from-to)186-194
Number of pages9
JournalSarcoidosis Vasculitis and Diffuse Lung Diseases
Volume22
Issue number3
Publication statusPublished - Oct 2005

Fingerprint

Pulmonary Sarcoidosis
Bronchoalveolar Lavage Fluid
Nerve Growth Factors
Therapeutic Irrigation
Alveolar Macrophages
Brain-Derived Neurotrophic Factor
Nerve Growth Factor
Neurotrophin 3
Nerve Growth Factor Receptors
T-Lymphocytes
Sarcoidosis
trkC Receptor
trkA Receptor
trkB Receptor
Chronic Granulomatous Disease
Lymphocytosis
CD4-CD8 Ratio
Immune System Diseases
Disease Progression
Molecular Biology

Keywords

  • Immunocytochemistry
  • Lymphocytes
  • Macrophages
  • Molecular biology
  • Neurotrophins

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Ricci, A., Mariotta, S., Saltini, C., Falasca, C., Giovagnoli, M. R., Mannino, F., ... Amenta, F. (2005). Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis. Sarcoidosis Vasculitis and Diffuse Lung Diseases, 22(3), 186-194.

Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis. / Ricci, Alberto; Mariotta, Salvatore; Saltini, Cesare; Falasca, Carlo; Giovagnoli, Maria Rosaria; Mannino, Francesco; Graziano, Paolo; Sciacchitano, Salvatore; Amenta, Francesco.

In: Sarcoidosis Vasculitis and Diffuse Lung Diseases, Vol. 22, No. 3, 10.2005, p. 186-194.

Research output: Contribution to journalArticle

Ricci, A, Mariotta, S, Saltini, C, Falasca, C, Giovagnoli, MR, Mannino, F, Graziano, P, Sciacchitano, S & Amenta, F 2005, 'Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis', Sarcoidosis Vasculitis and Diffuse Lung Diseases, vol. 22, no. 3, pp. 186-194.
Ricci A, Mariotta S, Saltini C, Falasca C, Giovagnoli MR, Mannino F et al. Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis. Sarcoidosis Vasculitis and Diffuse Lung Diseases. 2005 Oct;22(3):186-194.
Ricci, Alberto ; Mariotta, Salvatore ; Saltini, Cesare ; Falasca, Carlo ; Giovagnoli, Maria Rosaria ; Mannino, Francesco ; Graziano, Paolo ; Sciacchitano, Salvatore ; Amenta, Francesco. / Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis. In: Sarcoidosis Vasculitis and Diffuse Lung Diseases. 2005 ; Vol. 22, No. 3. pp. 186-194.
@article{455b1666dfbb40e1ac6abd66b34168ec,
title = "Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis",
abstract = "Background and aim of the study: Pulmonary sarcoidosis is a chronic granulomatous disease characterized by macrophage and CD4+ T-cell accumulation at the site of inflammation. Analysis of the cytokine network has substantially improved knowledge on immunopathogenesis of sarcoidosis. We hypothesize that neurotrophins (NTs), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and NT-3, besides their importance in immune system activities, participate in chronic inflammatory disorders and in repair processes. Methods: The expression of NTs and NT receptors was assessed in broncho alveolar lavage (BAL) macrophages, CD4 and CD8 T-cells, from 10 patients with pulmonary sarcoidosis, using molecular biology, Western blotting and immunocytochemistry. Results: Increased levels of NTs and of high affinity NT receptor (Trks) transcripts and proteins in BAL macrophages, CD4+ and CD8+ T-cells from pulmonary sarcoidosis patients were demonstrated in comparison with healthy controls. Contrarily to healthy controls, in pulmonary sarcoidosis the expression of NGF was increased in alveolar macrophages as well as NGF and BDNF in CD4+ and CD8+ T-cells. An increased expression of TrkA, TrkB and TrkC receptors was also noticeable. Furthermore, BDNF expression in alveolar macrophages and NT-3 expression in the three different BAL immune cell populations investigated were induced during sarcoidosis. A significant correlation was observed between CD4:CD8 ratio, lymphocytosis, radiological stage and CD4 and CD8 NT expression. Conclusions: These findings suggest that NTs are exaggeratedly expressed in BAL immune cells in pulmonary sarcoidosis and may participate in the progression of disease modulating immune cell functions.",
keywords = "Immunocytochemistry, Lymphocytes, Macrophages, Molecular biology, Neurotrophins",
author = "Alberto Ricci and Salvatore Mariotta and Cesare Saltini and Carlo Falasca and Giovagnoli, {Maria Rosaria} and Francesco Mannino and Paolo Graziano and Salvatore Sciacchitano and Francesco Amenta",
year = "2005",
month = "10",
language = "English",
volume = "22",
pages = "186--194",
journal = "Sarcoidosis Vasculitis and Diffuse Lung Diseases",
issn = "1124-0490",
publisher = "Mattioli 1885 S.p.A.",
number = "3",

}

TY - JOUR

T1 - Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in pulmonary sarcoidosis

AU - Ricci, Alberto

AU - Mariotta, Salvatore

AU - Saltini, Cesare

AU - Falasca, Carlo

AU - Giovagnoli, Maria Rosaria

AU - Mannino, Francesco

AU - Graziano, Paolo

AU - Sciacchitano, Salvatore

AU - Amenta, Francesco

PY - 2005/10

Y1 - 2005/10

N2 - Background and aim of the study: Pulmonary sarcoidosis is a chronic granulomatous disease characterized by macrophage and CD4+ T-cell accumulation at the site of inflammation. Analysis of the cytokine network has substantially improved knowledge on immunopathogenesis of sarcoidosis. We hypothesize that neurotrophins (NTs), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and NT-3, besides their importance in immune system activities, participate in chronic inflammatory disorders and in repair processes. Methods: The expression of NTs and NT receptors was assessed in broncho alveolar lavage (BAL) macrophages, CD4 and CD8 T-cells, from 10 patients with pulmonary sarcoidosis, using molecular biology, Western blotting and immunocytochemistry. Results: Increased levels of NTs and of high affinity NT receptor (Trks) transcripts and proteins in BAL macrophages, CD4+ and CD8+ T-cells from pulmonary sarcoidosis patients were demonstrated in comparison with healthy controls. Contrarily to healthy controls, in pulmonary sarcoidosis the expression of NGF was increased in alveolar macrophages as well as NGF and BDNF in CD4+ and CD8+ T-cells. An increased expression of TrkA, TrkB and TrkC receptors was also noticeable. Furthermore, BDNF expression in alveolar macrophages and NT-3 expression in the three different BAL immune cell populations investigated were induced during sarcoidosis. A significant correlation was observed between CD4:CD8 ratio, lymphocytosis, radiological stage and CD4 and CD8 NT expression. Conclusions: These findings suggest that NTs are exaggeratedly expressed in BAL immune cells in pulmonary sarcoidosis and may participate in the progression of disease modulating immune cell functions.

AB - Background and aim of the study: Pulmonary sarcoidosis is a chronic granulomatous disease characterized by macrophage and CD4+ T-cell accumulation at the site of inflammation. Analysis of the cytokine network has substantially improved knowledge on immunopathogenesis of sarcoidosis. We hypothesize that neurotrophins (NTs), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and NT-3, besides their importance in immune system activities, participate in chronic inflammatory disorders and in repair processes. Methods: The expression of NTs and NT receptors was assessed in broncho alveolar lavage (BAL) macrophages, CD4 and CD8 T-cells, from 10 patients with pulmonary sarcoidosis, using molecular biology, Western blotting and immunocytochemistry. Results: Increased levels of NTs and of high affinity NT receptor (Trks) transcripts and proteins in BAL macrophages, CD4+ and CD8+ T-cells from pulmonary sarcoidosis patients were demonstrated in comparison with healthy controls. Contrarily to healthy controls, in pulmonary sarcoidosis the expression of NGF was increased in alveolar macrophages as well as NGF and BDNF in CD4+ and CD8+ T-cells. An increased expression of TrkA, TrkB and TrkC receptors was also noticeable. Furthermore, BDNF expression in alveolar macrophages and NT-3 expression in the three different BAL immune cell populations investigated were induced during sarcoidosis. A significant correlation was observed between CD4:CD8 ratio, lymphocytosis, radiological stage and CD4 and CD8 NT expression. Conclusions: These findings suggest that NTs are exaggeratedly expressed in BAL immune cells in pulmonary sarcoidosis and may participate in the progression of disease modulating immune cell functions.

KW - Immunocytochemistry

KW - Lymphocytes

KW - Macrophages

KW - Molecular biology

KW - Neurotrophins

UR - http://www.scopus.com/inward/record.url?scp=27944470589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27944470589&partnerID=8YFLogxK

M3 - Article

C2 - 16315781

AN - SCOPUS:27944470589

VL - 22

SP - 186

EP - 194

JO - Sarcoidosis Vasculitis and Diffuse Lung Diseases

JF - Sarcoidosis Vasculitis and Diffuse Lung Diseases

SN - 1124-0490

IS - 3

ER -