Neurotrophin system activation in pleural effusions

Alberto Ricci, Salvatore Mariotta, Elena Pompili, Rita Mancini, Elena Bronzetti, Claudia De Vitis, Lara Pisani, Emanuela Cherubini, Pierdonato Bruno, Giorgetta Gencarelli, Maria R. Giovagnoli, Claudio Terzano, Gennaro Ciliberto, Enrico Giarnieri, Lorenzo Fumagalli

Research output: Contribution to journalArticle

Abstract

Neurotrophins (NTs) expression was assessed in malignant and non-malignant pleural effusions (inflammatory exudates and transudates). Enzyme-linked immunosorbent assay, in malignant exudates from small and non-small cell lung cancer (SCLC and NSCLC), detected nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3), and their levels are higher as compared with inflammatory and transudative effusions. By immunoblots, in cultured cancer cells coming from malignant pleural effusions, NTs and low-and high-affinity NT receptors were detected in a percentage of SCLC and NSCLC. Proliferation assay demonstrated that BDNF significantly increased cancer cell proliferation in vitro, on the contrary, NT-3 reduced cancer cell growth rate and NGF did not modify cell growth. Moreover, NGF protects cells from death during starvation. These effects are reverted by the addition of NT receptor antagonists. Cultured cancer cells injected into the lung of immunodeficient mice generate lung tumors expressing NTs and NT receptors. These findings suggest that NTs may be able to modulate cancer cell behavior and their growth.

Original languageEnglish
Pages (from-to)221-231
Number of pages11
JournalGrowth Factors
Volume28
Issue number4
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Human
  • Lung cancer
  • Neurotrophins
  • Nod Scid mice
  • Pleural effusion
  • Receptors

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology
  • Cell Biology

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  • Cite this

    Ricci, A., Mariotta, S., Pompili, E., Mancini, R., Bronzetti, E., De Vitis, C., Pisani, L., Cherubini, E., Bruno, P., Gencarelli, G., Giovagnoli, M. R., Terzano, C., Ciliberto, G., Giarnieri, E., & Fumagalli, L. (2010). Neurotrophin system activation in pleural effusions. Growth Factors, 28(4), 221-231. https://doi.org/10.3109/08977191003677402