Neutrophil extracellular traps mediate transfer of cytoplasmic neutrophil antigens to myeloid dendritic cells toward ANCA induction and associated autoimmunity

Sabina Sangaletti, Claudio Tripodo, Claudia Chiodoni, Carla Guarnotta, Barbara Cappetti, Patrizia Casalini, Silvia Piconese, Mariella Parenza, Cristiana Guiducci, Caterina Vitali, Mario P. Colombo

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Antineutrophil cytoplasmic antibodies (ANCAs) target proteins normally retained within neutrophils, indicating that cell death is involved in the autoimmunity process. Still, ANCA pathogenesis remains obscure. ANCAs activate neutrophils inducing their respiratory burst and a peculiar form of cell death, named NETosis, characterized by formation of neutrophil extracellular traps (NETs), decondensed chromatin threads decorated with cytoplasmic proteins endorsed with antimicrobial activity. NETs have been consistently detected in ANCA-associated small-vessel vasculitis, and this association prompted us to test whether the peculiar structure of NET favors neutrophil proteins uploading into myeloid dendritic cells and the induction of ANCAs and associated autoimmunity. Here we show that myeloid DCs uploaded with and activated by NET components induce ANCA and autoimmunity when injected into naive mice. DC uploading and autoimmunity induction are prevented by NET treatment with DNAse, indicating that NET structural integrity is needed to maintain the antigenicity of cytoplasmic proteins. We found NET intermingling with myeloid dendritic cells also positive for neutrophil myeloperoxidase in myeloperoxidase-ANCA-associated microscopic poliangiitis providing a potential correlative picture in human pathology. These data provide the first demonstration that NET structures are highly immunogenic such to trigger adaptive immune response relevant for autoimmunity.

Original languageEnglish
Pages (from-to)3007-3018
Number of pages12
JournalBlood
Volume120
Issue number15
DOIs
Publication statusPublished - Oct 11 2012

Fingerprint

Antineutrophil Cytoplasmic Antibodies
Myeloid Cells
Autoimmunity
Dendritic Cells
Neutrophils
Antigens
Cell death
Peroxidase
Proteins
Cell Death
Structural integrity
Pathology
Respiratory Burst
Extracellular Traps
Adaptive Immunity
Chromatin
Vasculitis
Demonstrations

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Neutrophil extracellular traps mediate transfer of cytoplasmic neutrophil antigens to myeloid dendritic cells toward ANCA induction and associated autoimmunity. / Sangaletti, Sabina; Tripodo, Claudio; Chiodoni, Claudia; Guarnotta, Carla; Cappetti, Barbara; Casalini, Patrizia; Piconese, Silvia; Parenza, Mariella; Guiducci, Cristiana; Vitali, Caterina; Colombo, Mario P.

In: Blood, Vol. 120, No. 15, 11.10.2012, p. 3007-3018.

Research output: Contribution to journalArticle

Sangaletti, S, Tripodo, C, Chiodoni, C, Guarnotta, C, Cappetti, B, Casalini, P, Piconese, S, Parenza, M, Guiducci, C, Vitali, C & Colombo, MP 2012, 'Neutrophil extracellular traps mediate transfer of cytoplasmic neutrophil antigens to myeloid dendritic cells toward ANCA induction and associated autoimmunity', Blood, vol. 120, no. 15, pp. 3007-3018. https://doi.org/10.1182/blood-2012-03-416156
Sangaletti, Sabina ; Tripodo, Claudio ; Chiodoni, Claudia ; Guarnotta, Carla ; Cappetti, Barbara ; Casalini, Patrizia ; Piconese, Silvia ; Parenza, Mariella ; Guiducci, Cristiana ; Vitali, Caterina ; Colombo, Mario P. / Neutrophil extracellular traps mediate transfer of cytoplasmic neutrophil antigens to myeloid dendritic cells toward ANCA induction and associated autoimmunity. In: Blood. 2012 ; Vol. 120, No. 15. pp. 3007-3018.
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