TY - JOUR
T1 - Neutrophil-mediated cellular cytotoxicity induced by zymosan-activated serum
AU - Dallegri, F.
AU - Frumento, G.
AU - Maggi, A. M.
AU - Ballestrero, A.
AU - Muttini, P.
AU - Patrone, F.
PY - 1984
Y1 - 1984
N2 - Human neutrophils, incubated in the presence of zymosan-activated serum (ZAS), were able to damage ox red blood cells (ORBC) as assessed by a 4-hr 51Cr-release assay. Cells from a patient with chronic granulomatous disease were incapable of mediating ZAS-induced cytolysis. Cytotoxicity by normal neutrophils was prevented by N-ethyl-maleimide as well as by catalase, but heat-inactivated catalase and superoxide dismutase were completely ineffective. Heme enzyme inhibitors, azide and cyanide, only partially reduced the target cell lysis. The results indicate the involvement of products of the oxidative metabolism, mainly hydrogen peroxide, as cytolytic mediators. These mediators released by neutrophils may contribute to the tissue injury occurring during inflammation.
AB - Human neutrophils, incubated in the presence of zymosan-activated serum (ZAS), were able to damage ox red blood cells (ORBC) as assessed by a 4-hr 51Cr-release assay. Cells from a patient with chronic granulomatous disease were incapable of mediating ZAS-induced cytolysis. Cytotoxicity by normal neutrophils was prevented by N-ethyl-maleimide as well as by catalase, but heat-inactivated catalase and superoxide dismutase were completely ineffective. Heme enzyme inhibitors, azide and cyanide, only partially reduced the target cell lysis. The results indicate the involvement of products of the oxidative metabolism, mainly hydrogen peroxide, as cytolytic mediators. These mediators released by neutrophils may contribute to the tissue injury occurring during inflammation.
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M3 - Article
C2 - 6726787
AN - SCOPUS:0021353149
VL - 13
SP - 89
EP - 92
JO - Journal of Clinical and Laboratory Immunology
JF - Journal of Clinical and Laboratory Immunology
SN - 0141-2760
IS - 2
ER -