TY - JOUR
T1 - Neutrophil-mediated cellular cytotoxicity triggered by immobilized aggregated IgG
T2 - An in vitro model of cell injury during immune complex diseases
AU - Dallegri, Franco
AU - Patrone, Franco
AU - Frumento, Guido
AU - Ballestrero, Alberto
AU - Sacchetti, Carlo
PY - 1984/11
Y1 - 1984/11
N2 - Normal human neutrophils were found to destroy ox red blood-cell targets when incubated on micropore filters coated with aggregated IgG, as determined by the51Cr release method. An intact neutrophil oxidative metabolism was essential for the cytotoxic event, since cells from patients with chronic granulomatous disease failed to exert any cytolysis. The target-cell destruction was prevented by catalase, azide, and cyanide and was enhanced by superoxide dismutase, suggesting involvement of the myeloperoxidase-hydrogen peroxide system. Neutrophil-mediated cytotoxicity was markedly amplified by the chemotactic peptide N-formyl-methionyl-leucylphenylalanine, as a result of an increased activity of the myeloperoxidase-hydrogen peroxide cytolytic system itself. This system of cytotoxicity provides a direct evidence for the neutrophil capacity of destroying bystander target cells under conditions simulating the in vivo immunologically mediated tissue injury and offers an excellent model to study events occurring during immune complex diseases.
AB - Normal human neutrophils were found to destroy ox red blood-cell targets when incubated on micropore filters coated with aggregated IgG, as determined by the51Cr release method. An intact neutrophil oxidative metabolism was essential for the cytotoxic event, since cells from patients with chronic granulomatous disease failed to exert any cytolysis. The target-cell destruction was prevented by catalase, azide, and cyanide and was enhanced by superoxide dismutase, suggesting involvement of the myeloperoxidase-hydrogen peroxide system. Neutrophil-mediated cytotoxicity was markedly amplified by the chemotactic peptide N-formyl-methionyl-leucylphenylalanine, as a result of an increased activity of the myeloperoxidase-hydrogen peroxide cytolytic system itself. This system of cytotoxicity provides a direct evidence for the neutrophil capacity of destroying bystander target cells under conditions simulating the in vivo immunologically mediated tissue injury and offers an excellent model to study events occurring during immune complex diseases.
KW - cytotoxicity
KW - Immune complex disease
KW - neutrophils
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U2 - 10.1007/BF00916573
DO - 10.1007/BF00916573
M3 - Article
C2 - 6096393
AN - SCOPUS:0021679776
VL - 4
SP - 439
EP - 444
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
SN - 0271-9142
IS - 6
ER -