Neutrophil-mediated cellular cytotoxicity triggered by immobilized aggregated IgG: An in vitro model of cell injury during immune complex diseases

Franco Dallegri, Franco Patrone, Guido Frumento, Alberto Ballestrero, Carlo Sacchetti

Research output: Contribution to journalArticlepeer-review

Abstract

Normal human neutrophils were found to destroy ox red blood-cell targets when incubated on micropore filters coated with aggregated IgG, as determined by the51Cr release method. An intact neutrophil oxidative metabolism was essential for the cytotoxic event, since cells from patients with chronic granulomatous disease failed to exert any cytolysis. The target-cell destruction was prevented by catalase, azide, and cyanide and was enhanced by superoxide dismutase, suggesting involvement of the myeloperoxidase-hydrogen peroxide system. Neutrophil-mediated cytotoxicity was markedly amplified by the chemotactic peptide N-formyl-methionyl-leucylphenylalanine, as a result of an increased activity of the myeloperoxidase-hydrogen peroxide cytolytic system itself. This system of cytotoxicity provides a direct evidence for the neutrophil capacity of destroying bystander target cells under conditions simulating the in vivo immunologically mediated tissue injury and offers an excellent model to study events occurring during immune complex diseases.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalJournal of Clinical Immunology
Volume4
Issue number6
DOIs
Publication statusPublished - Nov 1984

Keywords

  • cytotoxicity
  • Immune complex disease
  • neutrophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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