Neutrophilic cells in sputum of allergic asthmatic children

M. Miraglia Del Giudice, M. Pedullà, F. P. Brunese, A. F. Capristo, C. Capristo, M. A. Tosca, S. Leonardi, Giorgio Ciprandi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Airway inflammation is regarded as a central feature of asthma and is mostly sustained by eosinophilic infiltrate. Recent studies have shown that a co-activation of eosinophil- and neutrophil-dependent inflammatory mechanisms might explain why some asthmatics do not respond to conventional asthma therapy. The aim of our study is to determine whether neutrophilic inflammation was involved in 55 allergic children with mild-moderate persistent asthma and the relationship with the response to steroid treatment. Before the sputum analysis, all children underwent spirometry with the reversibility test, and were divided into two groups on the basis of the response (such as >12% of baseline FEV1): group 1 positive and group 2 negative. Eosinophil cationic protein concentrations were measured by radioimmunoassay and neutrophyl myeloperoxidase (MPO) concentrations were measured by an MPO-EIA. Ten healthy children of comparable ages served as control group. Total IgE, FEV1 and FEV/FVC values were similar in both groups. The sputum macrophage count was higher in controls than in allergic asthmatics, but there was no difference between groups 1 and 2 (59.6% vs 18.3% and 17%; p≤ 0.005). Sputum neutrophils were significantly higher in group 2 both vs controls (62% vs 34%; p≤ 0.005) and vs group 1 (62% vs 37%; p≤ 0.005). Our data suggest that neutrophils are involved in airway allergic inflammation in mild-moderate persistent childhood asthma and a high neutrophil count in sputum may be related to a lower responsiveness to inhaled corticosteroids.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalEuropean Journal of Inflammation
Volume8
Issue number3
Publication statusPublished - Sep 2010

Fingerprint

Sputum
Asthma
Neutrophils
Inflammation
Peroxidase
Eosinophil Cationic Protein
Neutrophil Activation
Spirometry
Eosinophils
Immunoglobulin E
Radioimmunoassay
Adrenal Cortex Hormones
Macrophages
Steroids
Control Groups
Therapeutics

Keywords

  • Atopic asthma
  • Induced sputum
  • Neutrophilic inflammation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Del Giudice, M. M., Pedullà, M., Brunese, F. P., Capristo, A. F., Capristo, C., Tosca, M. A., ... Ciprandi, G. (2010). Neutrophilic cells in sputum of allergic asthmatic children. European Journal of Inflammation, 8(3), 151-156.

Neutrophilic cells in sputum of allergic asthmatic children. / Del Giudice, M. Miraglia; Pedullà, M.; Brunese, F. P.; Capristo, A. F.; Capristo, C.; Tosca, M. A.; Leonardi, S.; Ciprandi, Giorgio.

In: European Journal of Inflammation, Vol. 8, No. 3, 09.2010, p. 151-156.

Research output: Contribution to journalArticle

Del Giudice, MM, Pedullà, M, Brunese, FP, Capristo, AF, Capristo, C, Tosca, MA, Leonardi, S & Ciprandi, G 2010, 'Neutrophilic cells in sputum of allergic asthmatic children', European Journal of Inflammation, vol. 8, no. 3, pp. 151-156.
Del Giudice MM, Pedullà M, Brunese FP, Capristo AF, Capristo C, Tosca MA et al. Neutrophilic cells in sputum of allergic asthmatic children. European Journal of Inflammation. 2010 Sep;8(3):151-156.
Del Giudice, M. Miraglia ; Pedullà, M. ; Brunese, F. P. ; Capristo, A. F. ; Capristo, C. ; Tosca, M. A. ; Leonardi, S. ; Ciprandi, Giorgio. / Neutrophilic cells in sputum of allergic asthmatic children. In: European Journal of Inflammation. 2010 ; Vol. 8, No. 3. pp. 151-156.
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