Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity

Giuseppina Blanca; Fausto Baldanti; Stefania Paolucci; Alexander Y. Skoblov; Lyubov Victorova; Ulrich Hübscher; Giuseppe Gerna; Silvio Spadari; Giovanni Maga

doi:10.1074/jbc.C300022200

Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity

Giuseppina Blanca, Fausto Baldanti, Stefania Paolucci, Alexander Y. Skoblov, Lyubov Victorova, Ulrich Hübscher, Giuseppe Gerna, Silvio Spadari, Giovanni Maga

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Recombinant HIV-1 reverse transcriptase (RT) carrying non-nucleoside inhibitors (NNRTIs) resistance mutation at codon 181 showed reduced incorporation and high efficiency of phosphorolytic removal of stavudine, a nucleoside RT inhibitor. These results reveal a new mechanism for cross-resistance between different classes of HIV-1 RT inhibitors.

Original language	English
Pages (from-to)	15469-15472
Number of pages	4
Journal	Journal of Biological Chemistry
Volume	278
Issue number	18
DOIs	https://doi.org/10.1074/jbc.C300022200
Publication status	Published - May 2 2003

ASJC Scopus subject areas

Biochemistry

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Blanca, G., Baldanti, F., Paolucci, S., Skoblov, A. Y., Victorova, L., Hübscher, U., Gerna, G., Spadari, S., & Maga, G. (2003). Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity. Journal of Biological Chemistry, 278(18), 15469-15472. https://doi.org/10.1074/jbc.C300022200

Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity. / Blanca, Giuseppina; Baldanti, Fausto ; Paolucci, Stefania; Skoblov, Alexander Y.; Victorova, Lyubov; Hübscher, Ulrich; Gerna, Giuseppe; Spadari, Silvio; Maga, Giovanni.

In: Journal of Biological Chemistry, Vol. 278, No. 18, 02.05.2003, p. 15469-15472.

Research output: Contribution to journal › Article › peer-review

Blanca, G, Baldanti, F , Paolucci, S, Skoblov, AY, Victorova, L, Hübscher, U, Gerna, G, Spadari, S & Maga, G 2003, 'Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity', Journal of Biological Chemistry, vol. 278, no. 18, pp. 15469-15472. https://doi.org/10.1074/jbc.C300022200

Blanca, Giuseppina ; Baldanti, Fausto ; Paolucci, Stefania ; Skoblov, Alexander Y. ; Victorova, Lyubov ; Hübscher, Ulrich ; Gerna, Giuseppe ; Spadari, Silvio ; Maga, Giovanni. / Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 18. pp. 15469-15472.

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abstract = "Recombinant HIV-1 reverse transcriptase (RT) carrying non-nucleoside inhibitors (NNRTIs) resistance mutation at codon 181 showed reduced incorporation and high efficiency of phosphorolytic removal of stavudine, a nucleoside RT inhibitor. These results reveal a new mechanism for cross-resistance between different classes of HIV-1 RT inhibitors.",

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AU - Blanca, Giuseppina

AU - Baldanti, Fausto

AU - Paolucci, Stefania

AU - Skoblov, Alexander Y.

AU - Victorova, Lyubov

AU - Hübscher, Ulrich

AU - Gerna, Giuseppe

AU - Spadari, Silvio

AU - Maga, Giovanni

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Nevirapine resistance mutation at codon 181 of the HIV-1 reverse transcriptase confers stavudine resistance by increasing nucleotide substrate discrimination and phosphorolytic activity

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