New aspects and perspectives in AIDS diagnosis

J. L. McDermott, A. A. Giri, M. Giacomini, D. Pietrapiana, I. Martini, F. Indiveri, F. Puppo, A. Campelli, L. Tagliaferro, A. Cara, O. E. Varnier

Research output: Contribution to journalArticle

Abstract

Discoveries on the efficacy of antiretroviral drugs have changed the management of HIV patients. More information on viral load is needed with accurate markers of remission and/or eradication. First objective: to evaluate the sensitivity of an innovative protocol for p24 antigen (3 Plus). The p24 assay has a sensitivity of ~70 fg/reaction, correlates with vital replication, viral load, CD4+ cell numbers and therapy efficacy. Thus it is a useful and inexpensive marker in AIDS management. Second objective: to develop and evaluate assays for total HIV DNA and non-integrated circular DNA. Total HIV DNA was detected in 96.9% of 548 samples from HIV-infected individuals. High levels of total HIV DNA in sequential samples correlated with therapy inefficacy and subsequent HIV replication, confirmed by the detection of nonintegrated DNA. In absence of NI-DNA, total HIV DNA contains only proviral DNA and is the only marker of remission and/or eradication.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalAlpe Adria Microbiology Journal
Volume8
Issue number1
Publication statusPublished - 1999

Keywords

  • AIDS
  • DNA PCR
  • HIV
  • p24 antigen

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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    McDermott, J. L., Giri, A. A., Giacomini, M., Pietrapiana, D., Martini, I., Indiveri, F., Puppo, F., Campelli, A., Tagliaferro, L., Cara, A., & Varnier, O. E. (1999). New aspects and perspectives in AIDS diagnosis. Alpe Adria Microbiology Journal, 8(1), 49-53.