New cross-talk layer between ultraconserved non-coding RNAs, microRNAs and polycomb protein YY1 in bladder cancer

Sara Terreri, Montano Durso, Vincenza Colonna, Alessandra Romanelli, Daniela Terracciano, Matteo Ferro, Sisto Perdonà, Luigi Castaldo, Ferdinando Febbraio, Filomena de Nigris, Amelia Cimmino

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) are highly conserved elements in mammals, and exert key regulatory functions. Growing evidence shows that miRNAs can interact with another class of non-coding RNAs, so-called transcribed ultraconserved regions (T-UCRs), which take part in transcriptional, post-transcriptional and epigenetic regulation processes. We report here the interaction of miRNAs and T-UCRs as a network modulating the availability of these non-coding RNAs in bladder cancer cells. In our cell system, antagomiR-596 increased the expression of T-UCR 201+. Moreover, T-UCR 8+ silencing increased miR-596 expression, which in turn reduced total T-UCR 283+, showing that the perturbation of one element in this network changes the expression of other interactors. In addition, we identify the polycomb protein Yin Yang 1 (YY1) as mediator of binding between miR-596 and T-UCR 8+. These new findings describe for the first time a network between T-UCRs, miRNAs and YY1 protein, highlighting the existence of an additional layer of gene expression regulation.

Original languageEnglish
Article number127
JournalGenes
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 14 2016

Keywords

  • Bladder cancer
  • Interaction network
  • MicroRNAs
  • Transcribed ultraconserved regions

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Terreri, S., Durso, M., Colonna, V., Romanelli, A., Terracciano, D., Ferro, M., Perdonà, S., Castaldo, L., Febbraio, F., de Nigris, F., & Cimmino, A. (2016). New cross-talk layer between ultraconserved non-coding RNAs, microRNAs and polycomb protein YY1 in bladder cancer. Genes, 7(12), [127]. https://doi.org/10.3390/genes7120127