TY - JOUR
T1 - New era for pancreatic endoscopic ultrasound
T2 - From imaging to molecular pathology of pancreatic cancer
AU - Archibugi, Livia
AU - Testoni, Sabrina Gloria Giulia
AU - Redegalli, Miriam
AU - Petrone, Maria Chiara
AU - Reni, Michele
AU - Falconi, Massimo
AU - Doglioni, Claudio
AU - Capurso, Gabriele
AU - Arcidiacono, Paolo Giorgio
PY - 2019/11/1
Y1 - 2019/11/1
N2 - With recent advances in molecular pathology and the development of new chemotherapy regimens, the knowledge of the molecular alterations of pancreatic ductal adenocarcinoma (PDAC) is becoming appealing for stratifying patients for prognosis and response to a defined treatment. Archival formalin-fixed, paraffinembedded samples are a useful source of genomic deoxyribonucleic acid; nevertheless, most studies employed formalin-fixed, paraffin-embedded samples deriving from surgical specimens, which are therefore representative of <20% of PDAC patients. Indeed, the development of a reliable methodology for endoscopic ultrasound-guided tissue acquisition, stabilization, and analysis is crucial for the development of molecular markers for clinical use in order to achieve "personalized medicine". With the development of new needles, this technique is able to retrieve a high quantity and quality of PDAC tissue that can be used not only for diagnosis but also for mutational and transcriptome evaluations and for the development of primary cell or tissue cultures. In the present editorial, we discuss the current knowledge regarding the use of endoscopic ultrasound as a tool to obtain samples for molecular analyses, its possible pitfalls, and its use for the development of disease models such as xenografts or organoids.
AB - With recent advances in molecular pathology and the development of new chemotherapy regimens, the knowledge of the molecular alterations of pancreatic ductal adenocarcinoma (PDAC) is becoming appealing for stratifying patients for prognosis and response to a defined treatment. Archival formalin-fixed, paraffinembedded samples are a useful source of genomic deoxyribonucleic acid; nevertheless, most studies employed formalin-fixed, paraffin-embedded samples deriving from surgical specimens, which are therefore representative of <20% of PDAC patients. Indeed, the development of a reliable methodology for endoscopic ultrasound-guided tissue acquisition, stabilization, and analysis is crucial for the development of molecular markers for clinical use in order to achieve "personalized medicine". With the development of new needles, this technique is able to retrieve a high quantity and quality of PDAC tissue that can be used not only for diagnosis but also for mutational and transcriptome evaluations and for the development of primary cell or tissue cultures. In the present editorial, we discuss the current knowledge regarding the use of endoscopic ultrasound as a tool to obtain samples for molecular analyses, its possible pitfalls, and its use for the development of disease models such as xenografts or organoids.
KW - Deoxyribonucleic acid
KW - Endoscopic ultrasound
KW - Molecular
KW - Mutation
KW - Organoid
KW - Pancreatic cancer
KW - Personalized medicine
KW - Profiling
KW - Ribonucleic acid
UR - http://www.scopus.com/inward/record.url?scp=85076796759&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076796759&partnerID=8YFLogxK
U2 - 10.4251/wjgo.v11.i11.933
DO - 10.4251/wjgo.v11.i11.933
M3 - Editorial
AN - SCOPUS:85076796759
VL - 11
SP - 933
EP - 945
JO - World Journal of Gastrointestinal Oncology
JF - World Journal of Gastrointestinal Oncology
SN - 1948-5204
IS - 11
ER -