Abstract
Although magnesium ions play a key role in many fundamental biological processes, information about its intracellular regulation is still scarce, due to the lack of appropriate detection methods. Here, we report the spectroscopic characterization of two diaza-18-crown-6 hydroxyquinoline derivatives (DCHQ) and we propose their application for the determination of total Mg 2+ concentration and in confocal imaging as effective Mg 2+ indicators. DCHQ derivatives 1 and 2 bind Mg 2+ with much higher affinity than other available probes (K d = 44 and 73 mM, respectively) with a concomitant strong fluorescence increase. On the other hand, the fluorescence intensity is not significantly affected by other divalent cations, most importantly Ca 2+, or by pH changes within the physiological range. Evidence is provided on the use of fluorometric data to derive totalcellular Mg 2+ content, which is in agreement with atomic absorption data. Furthermore, we show that DCHQ compounds can be effectively employed to map intracellular ion distribution and movements in live cells by confocal microscopy. These findings suggest that DCHQ derivatives may serve as new probes for the study of Mg 2+ regulation, allowing sensitive and straightforward detection of both static and dynamic signals.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6097 |
| DOIs | |
| Publication status | Published - 2006 |
| Event | Optical Molecular Probes for Biomedical Applications - San Jose, CA, United States Duration: Jan 22 2006 → Jan 24 2006 |
Other
| Other | Optical Molecular Probes for Biomedical Applications |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/22/06 → 1/24/06 |
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Keywords
- Fluorescent probes
- Intracellular concentration
- Luminescence
- Magnesium ion
- Photophysical properties
ASJC Scopus subject areas
- Engineering(all)
Cite this
New fluorescent chemosensors for magnesium ions in living cells. / Prodi, L.; Dolci, L. S.; Montalti, M.; Zaccheroni, N.; Farruggia, G.; Andreani, G.; Carpenè, E.; Iotti, S.; Savage, P. B.; Trapani, V.; Sale, P.; Wolf, F. I.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6097 2006. 60970C.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - New fluorescent chemosensors for magnesium ions in living cells
AU - Prodi, L.
AU - Dolci, L. S.
AU - Montalti, M.
AU - Zaccheroni, N.
AU - Farruggia, G.
AU - Andreani, G.
AU - Carpenè, E.
AU - Iotti, S.
AU - Savage, P. B.
AU - Trapani, V.
AU - Sale, P.
AU - Wolf, F. I.
PY - 2006
Y1 - 2006
N2 - Although magnesium ions play a key role in many fundamental biological processes, information about its intracellular regulation is still scarce, due to the lack of appropriate detection methods. Here, we report the spectroscopic characterization of two diaza-18-crown-6 hydroxyquinoline derivatives (DCHQ) and we propose their application for the determination of total Mg 2+ concentration and in confocal imaging as effective Mg 2+ indicators. DCHQ derivatives 1 and 2 bind Mg 2+ with much higher affinity than other available probes (K d = 44 and 73 mM, respectively) with a concomitant strong fluorescence increase. On the other hand, the fluorescence intensity is not significantly affected by other divalent cations, most importantly Ca 2+, or by pH changes within the physiological range. Evidence is provided on the use of fluorometric data to derive totalcellular Mg 2+ content, which is in agreement with atomic absorption data. Furthermore, we show that DCHQ compounds can be effectively employed to map intracellular ion distribution and movements in live cells by confocal microscopy. These findings suggest that DCHQ derivatives may serve as new probes for the study of Mg 2+ regulation, allowing sensitive and straightforward detection of both static and dynamic signals.
AB - Although magnesium ions play a key role in many fundamental biological processes, information about its intracellular regulation is still scarce, due to the lack of appropriate detection methods. Here, we report the spectroscopic characterization of two diaza-18-crown-6 hydroxyquinoline derivatives (DCHQ) and we propose their application for the determination of total Mg 2+ concentration and in confocal imaging as effective Mg 2+ indicators. DCHQ derivatives 1 and 2 bind Mg 2+ with much higher affinity than other available probes (K d = 44 and 73 mM, respectively) with a concomitant strong fluorescence increase. On the other hand, the fluorescence intensity is not significantly affected by other divalent cations, most importantly Ca 2+, or by pH changes within the physiological range. Evidence is provided on the use of fluorometric data to derive totalcellular Mg 2+ content, which is in agreement with atomic absorption data. Furthermore, we show that DCHQ compounds can be effectively employed to map intracellular ion distribution and movements in live cells by confocal microscopy. These findings suggest that DCHQ derivatives may serve as new probes for the study of Mg 2+ regulation, allowing sensitive and straightforward detection of both static and dynamic signals.
KW - Fluorescent probes
KW - Intracellular concentration
KW - Luminescence
KW - Magnesium ion
KW - Photophysical properties
UR - http://www.scopus.com/inward/record.url?scp=33646176150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646176150&partnerID=8YFLogxK
U2 - 10.1117/12.645783
DO - 10.1117/12.645783
M3 - Conference contribution
AN - SCOPUS:33646176150
SN - 0819461393
SN - 9780819461391
VL - 6097
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -