New heparinizable modified poly(carbonate urethane) surfaces diminishing bacterial colonization

Luigi De Nardo, Silvia Farè, Valentina Di Matteo, Eliana Cipolla, Enrica Saino, Livia Visai, Pietro Speziale, Maria Cristina Tanzi

Research output: Contribution to journalArticlepeer-review

Abstract

Percutaneous devices are extensively used in modern medicine therapies, even in long term applications. Complications from their use, related to bacterial colonization and/or to materials thrombogenicity, may result in a significant morbidity and mortality incidence. In this study, a novel polycarbonate-urethane (PCU), incorporating a tailor-made diamino-diamide-diol (PIME) showing the ability to bind heparin at physiological pH, was compared to commercial medical-grade PCUs (Carbothane® and Bionate®). Mechanical and thermal properties were evaluated by tensile tests, dynamic mechanical analysis and differential scanning calorimetry. The presence of a low amount of PIME chain extender in Bionate® polyurethanes (Bionate-PIME) slightly affects the mechanical properties, remaining however comparable with the medical grade PCUs used for the fabrication of cardiovascular devices. To verify thereof heparin surface adsorbed in disfavouring bacterial colonization, heparinized Bionate-PIME was tested for bacterial adhesion, using Bionate® and Carbothane® as reference. In vitro bacterial interaction tests were performed with the strains mainly involved in the pathogenesis of device-related infections (S. epidermidis and S. aureus). MTT tests and SEM observations showed a decrease in colonization of the different strains on the heparinized Bionate-PIME surfaces, confirming that preadsorbed heparin plays a role in mediating the biomaterial surface/bacterial cells interactions.

Original languageEnglish
Pages (from-to)2109-2115
Number of pages7
JournalJournal of Materials Science: Materials in Medicine
Volume18
Issue number11
DOIs
Publication statusPublished - Nov 2007

ASJC Scopus subject areas

  • Biophysics
  • Chemical Engineering(all)
  • Bioengineering

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