New HLA class I-like alloantigens expressed on blast cells.

T. Crepaldi, D. Peruccio, M. Lecchi, L. Resegotti, M. Grazia Guerra, A. Funaro, P. Richiardi

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Abstract

New beta 2-microglobulin (beta 2-m)-associated, HLA-linked alloantigens, selectively expressed on phytohaemagglutinin (PHA)-activated lymphocytes, were identified using human alloantisera. Reactivity of the antisera against activated cells correlated with HLA-A2, A10 and A28 specificities. The new alloantigens were undetectable on peripheral blood mononuclear cells, B lymphocytes and platelets using either the lymphocytotoxicity or the absorption techniques, and appeared on lymphocytes upon PHA activation, with time-dependent kinetics. They were found on some, but not all, acute leukaemias of B, T and myeloid origin, being absent from chronic B lymphocytic leukaemias. The presence of the new beta 2-microglobulin-associated allospecificities was not correlated with the quantity of classical class I antigens present, as analysed on different cell types by flow cytometry. Thus, these antigenic determinants appear to be different from the classical HLA class I antigens and could be the human counterpart of the murine Qa system or of the second H-2K gene.

Original languageEnglish
Pages (from-to)219-229
Number of pages11
JournalJournal of Immunogenetics
Volume14
Issue number4-5
Publication statusPublished - Aug 1987

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ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Crepaldi, T., Peruccio, D., Lecchi, M., Resegotti, L., Grazia Guerra, M., Funaro, A., & Richiardi, P. (1987). New HLA class I-like alloantigens expressed on blast cells. Journal of Immunogenetics, 14(4-5), 219-229.