New huntington disease mutation arising from a paternal CAG34 allele showing somatic length variation in serially passaged lymphoblasts

Milena Cannella, Vittorio Maglione, Tiziana Martino, Maria Simonelli, Giuseppe Ragona, Ferdinando Squitieri

Research output: Contribution to journalArticlepeer-review

Abstract

The analysis of somatic CAG triplet variation in lymphoblastoid cell lines from subjects carrying alleles of intermediate length (IA33CAG and IA34CAG) in Huntington disease (HD) gene disclosed instability in the DNA of the person, from whom a new expansion mutation of 45 triplets originated. The triplet size increased after about 30 passages in cell cultures in lymphoblasts with the IA34 genotype. Lymphoblasts may provide an appropriate model for studying repeat instability in subjects with poly(CAG) repeat disorders. HD shows somatic, in addition to germ-line instability, highlighting the propensity to somatic CAG variation in human cells even with repeat numbers under the expanded edge. Factors potentially cis acting with the mutation, other than those reported in this study (CCG polymorphic stretch, the deletion of the glutamic acid residue at position 2642 and the 4-codon segment between CAG and CCG polymorphisms), should be searched for and analyzed.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalAmerican Journal of Medical Genetics - Neuropsychiatric Genetics
Volume133 B
Issue number1
DOIs
Publication statusPublished - Feb 5 2005

Keywords

  • cis-acting factors
  • Intermediate alleles
  • New mutation
  • Somatic CAG repeat variation

ASJC Scopus subject areas

  • Genetics(clinical)
  • Neuropsychology and Physiological Psychology
  • Neuroscience(all)

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