New hydrogel for neuroglial cell housing

Filippo Rossi, Giuseppe Perale, Marta Tunesi, Carmen Giordano, Francesco Daniele, Fabio Bianco, Michela Matteoli, Maurizio Masi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hydrogels specifically suited for housing central nervous system (CNS) cells represent a weak minority within literature available on gels as tissue engineering scaffolds. This is due to difficulties in building hydrogels allowing CNS cells to survive within them. We aimed at developing a resorbable injectable hydrogel as a suitable cell housing vector for regenerative approaches, particularly to be used with neuroglial cells in injured spinal cord rewiring. New polymeric formulation was studied for hydrogel synthesis together with new protocols for building three-dimensional cell/hydrogel biohybrid constructs. Formulations are based on two different pharmaceutical-grade polymers (a synthetic carbomer and a natural agarose) together with specific crosslinking agents. Gelation procedure allows cells loading directly into construct during gelation (T6 cells per ml of gel. Hydrogels resulted biocompatible and resorbable: degradation patterns were compatible with cell survival and local tissue housing needs. Injectability was confirmed by tixotropic behaviour. Optical imaging assays showed healthy and spreading cells within gel at various time points; standard living cell counts performed after trypan colouring resulted in about 50% (±10%) of living cells within hydrogel matrix. Our hydrogels are thus among the few that can be used for building 3D constructs for neural cells carrying in reparatory approaches.

Original languageEnglish
Title of host publicationAES-ATEMA International Conference Series - Advances and Trends in Engineering Materials and their Applications
Pages227-231
Number of pages5
Publication statusPublished - 2009
Event3rd International Conference on Advances and Trends in Engineering Materials and their Applications, AES-ATEMA'2009 - Montreal, QC, Canada
Duration: Jul 6 2009Jul 10 2009

Other

Other3rd International Conference on Advances and Trends in Engineering Materials and their Applications, AES-ATEMA'2009
CountryCanada
CityMontreal, QC
Period7/6/097/10/09

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Keywords

  • Biohybrid devices
  • Hydrogel
  • Injectable gels
  • Neural cell housing
  • Neural tissue engineering
  • Regenerative medicine

ASJC Scopus subject areas

  • Mechanics of Materials
  • Materials Science(all)

Cite this

Rossi, F., Perale, G., Tunesi, M., Giordano, C., Daniele, F., Bianco, F., Matteoli, M., & Masi, M. (2009). New hydrogel for neuroglial cell housing. In AES-ATEMA International Conference Series - Advances and Trends in Engineering Materials and their Applications (pp. 227-231)