New insights in extracellular matrix remodeling and collagen turnover related pathways in cultured human tenocytes after ciprofloxacin administration

Alessandra Menon, Letizia Pettinari, Carla Martinelli, Graziano Colombo, Nicola Portinaro, Isabella Dalle-Donne, Maria Cristina D'Agostino, Nicoletta Gagliano

Research output: Contribution to journalArticlepeer-review

Abstract

We characterized the effect of ciprofloxacin (CPX) in cultured human tenocytes by morphological and molecular methods. Collagen type I and III mRNA and protein levels were unaffected, but lysyl hydroxylase 2b mRNA levels progressively decreased after CPX administration. MMP- 1 protein levels significantly increased after 20 μg/ml CPX administration but remained unmodified at the higher dose, whilst MMP-2 activity was unchanged. Tissue inhibitor of MMP (TIMP- 1) gene expression decreased after CPX treatment, whilst TIMP-2 and transforming growth factor-β1 gene expression, the cytoskeleton arrangement, and cytochrome c expression remained unmodified. Secreted Protein Acidic and Rich in Cysteine mRNA and protein levels remained almost unchanged, whilst N-cadherin mRNA levels resulted significantly down-regulated and connexin 43 gene expression tended to decrease after CPX administration. The CPX-induced decreased ability to cross-link collagen and decreased TIMP-1 levels, possibly leading to higher activity of MMPs in ECM degradation, together with the down-regulation of Ncadherin and connexin 43 are consistent with a reduced ability to maintain tissue homeostasis, possibly making the tendon more susceptible to rupture.

Original languageEnglish
Pages (from-to)122-131
Number of pages10
JournalMuscles, Ligaments and Tendons Journal
Volume3
Issue number3
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Ciprofloxacin
  • Collagen turnover
  • Extracellular matrix remodelling
  • Tendons
  • Tenocytes

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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