Abstract
Background and Purpose - The spontaneously hypertensive stroke-prone rat (SHRSP) is an animal model for a complex form of cerebrovascular pathology. MRI provides an efficient and noninvasive tool for studying the time course of brain damage. The aim of this study was to gain new insights into the pathological phenomena responsible for the occurrence of brain injury in SHRSP with the use of the apparent diffusion coefficient of water (ADC), one of the most efficient MRI parameters for detecting brain abnormalities. To this end, the pattern of ADC variation observed in SHRSP was compared with that of focal ischemia induced in both SHRSP and Sprague-Dawley rats. Methods - Four groups of animals were studied: SHRSP developing spontaneous brain lesions fed with a salt-loaded (n=15, group 1) or standard diet (n=3, group 2) and Sprague-Dawley rats (n=8, group 3) and SHRSP (n=8, group 4) with permanent middle cerebral artery occlusion. ADC maps and T2-weighted images of brains were performed by MRI. After the rats were killed, the brains were removed and histologically processed. Results - There was no decrease in ADC during spontaneous stroke in the SHRSP fed with a normal or salt-enriched diet, while both the SHRSP and Sprague-Dawley rats with middle cerebral artery occlusion showed a marked decrease that lasted for 24 to 48 hours. Conclusions - Cerebral ischemia cannot be considered a major factor in the onset of spontaneous brain lesions in SHRSP, which show only vasogenic edema after the beginning of the damage with no evidence of metabolic impairment.
| Original language | English |
|---|---|
| Pages (from-to) | 825-830 |
| Number of pages | 6 |
| Journal | Stroke |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2002 |
Fingerprint
Keywords
- Animal models
- Brain injuries
- Diffusion
- Magnetic resonance imaging
- Middle cerebral artery occlusion
- Rats
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Neuroscience(all)
Cite this
New insights into brain damage in stroke-prone rats : A nuclear magnetic imaging study. / Guerrini, Uliano; Sironi, Luigi; Tremoli, Elena; Cimino, Mauro; Pollo, Bianca; Calvio, Anna Maria; Paoletti, Rodolfo; Asdente, Maria.
In: Stroke, Vol. 33, No. 3, 2002, p. 825-830.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - New insights into brain damage in stroke-prone rats
T2 - A nuclear magnetic imaging study
AU - Guerrini, Uliano
AU - Sironi, Luigi
AU - Tremoli, Elena
AU - Cimino, Mauro
AU - Pollo, Bianca
AU - Calvio, Anna Maria
AU - Paoletti, Rodolfo
AU - Asdente, Maria
PY - 2002
Y1 - 2002
N2 - Background and Purpose - The spontaneously hypertensive stroke-prone rat (SHRSP) is an animal model for a complex form of cerebrovascular pathology. MRI provides an efficient and noninvasive tool for studying the time course of brain damage. The aim of this study was to gain new insights into the pathological phenomena responsible for the occurrence of brain injury in SHRSP with the use of the apparent diffusion coefficient of water (ADC), one of the most efficient MRI parameters for detecting brain abnormalities. To this end, the pattern of ADC variation observed in SHRSP was compared with that of focal ischemia induced in both SHRSP and Sprague-Dawley rats. Methods - Four groups of animals were studied: SHRSP developing spontaneous brain lesions fed with a salt-loaded (n=15, group 1) or standard diet (n=3, group 2) and Sprague-Dawley rats (n=8, group 3) and SHRSP (n=8, group 4) with permanent middle cerebral artery occlusion. ADC maps and T2-weighted images of brains were performed by MRI. After the rats were killed, the brains were removed and histologically processed. Results - There was no decrease in ADC during spontaneous stroke in the SHRSP fed with a normal or salt-enriched diet, while both the SHRSP and Sprague-Dawley rats with middle cerebral artery occlusion showed a marked decrease that lasted for 24 to 48 hours. Conclusions - Cerebral ischemia cannot be considered a major factor in the onset of spontaneous brain lesions in SHRSP, which show only vasogenic edema after the beginning of the damage with no evidence of metabolic impairment.
AB - Background and Purpose - The spontaneously hypertensive stroke-prone rat (SHRSP) is an animal model for a complex form of cerebrovascular pathology. MRI provides an efficient and noninvasive tool for studying the time course of brain damage. The aim of this study was to gain new insights into the pathological phenomena responsible for the occurrence of brain injury in SHRSP with the use of the apparent diffusion coefficient of water (ADC), one of the most efficient MRI parameters for detecting brain abnormalities. To this end, the pattern of ADC variation observed in SHRSP was compared with that of focal ischemia induced in both SHRSP and Sprague-Dawley rats. Methods - Four groups of animals were studied: SHRSP developing spontaneous brain lesions fed with a salt-loaded (n=15, group 1) or standard diet (n=3, group 2) and Sprague-Dawley rats (n=8, group 3) and SHRSP (n=8, group 4) with permanent middle cerebral artery occlusion. ADC maps and T2-weighted images of brains were performed by MRI. After the rats were killed, the brains were removed and histologically processed. Results - There was no decrease in ADC during spontaneous stroke in the SHRSP fed with a normal or salt-enriched diet, while both the SHRSP and Sprague-Dawley rats with middle cerebral artery occlusion showed a marked decrease that lasted for 24 to 48 hours. Conclusions - Cerebral ischemia cannot be considered a major factor in the onset of spontaneous brain lesions in SHRSP, which show only vasogenic edema after the beginning of the damage with no evidence of metabolic impairment.
KW - Animal models
KW - Brain injuries
KW - Diffusion
KW - Magnetic resonance imaging
KW - Middle cerebral artery occlusion
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=0036192442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036192442&partnerID=8YFLogxK
U2 - 10.1161/hs0302.104111
DO - 10.1161/hs0302.104111
M3 - Article
C2 - 11872910
AN - SCOPUS:0036192442
VL - 33
SP - 825
EP - 830
JO - Stroke
JF - Stroke
SN - 0039-2499
IS - 3
ER -