New insights into brain damage in stroke-prone rats: A nuclear magnetic imaging study

Uliano Guerrini, Luigi Sironi, Elena Tremoli, Mauro Cimino, Bianca Pollo, Anna Maria Calvio, Rodolfo Paoletti, Maria Asdente

Research output: Contribution to journalArticle

Abstract

Background and Purpose - The spontaneously hypertensive stroke-prone rat (SHRSP) is an animal model for a complex form of cerebrovascular pathology. MRI provides an efficient and noninvasive tool for studying the time course of brain damage. The aim of this study was to gain new insights into the pathological phenomena responsible for the occurrence of brain injury in SHRSP with the use of the apparent diffusion coefficient of water (ADC), one of the most efficient MRI parameters for detecting brain abnormalities. To this end, the pattern of ADC variation observed in SHRSP was compared with that of focal ischemia induced in both SHRSP and Sprague-Dawley rats. Methods - Four groups of animals were studied: SHRSP developing spontaneous brain lesions fed with a salt-loaded (n=15, group 1) or standard diet (n=3, group 2) and Sprague-Dawley rats (n=8, group 3) and SHRSP (n=8, group 4) with permanent middle cerebral artery occlusion. ADC maps and T2-weighted images of brains were performed by MRI. After the rats were killed, the brains were removed and histologically processed. Results - There was no decrease in ADC during spontaneous stroke in the SHRSP fed with a normal or salt-enriched diet, while both the SHRSP and Sprague-Dawley rats with middle cerebral artery occlusion showed a marked decrease that lasted for 24 to 48 hours. Conclusions - Cerebral ischemia cannot be considered a major factor in the onset of spontaneous brain lesions in SHRSP, which show only vasogenic edema after the beginning of the damage with no evidence of metabolic impairment.

Original languageEnglish
Pages (from-to)825-830
Number of pages6
JournalStroke
Volume33
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Stroke
Brain
Sprague Dawley Rats
Water
Middle Cerebral Artery Infarction
Salts
Diet
Brain Ischemia
Brain Injuries
Edema
Ischemia
Animal Models
Pathology

Keywords

  • Animal models
  • Brain injuries
  • Diffusion
  • Magnetic resonance imaging
  • Middle cerebral artery occlusion
  • Rats

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

New insights into brain damage in stroke-prone rats : A nuclear magnetic imaging study. / Guerrini, Uliano; Sironi, Luigi; Tremoli, Elena; Cimino, Mauro; Pollo, Bianca; Calvio, Anna Maria; Paoletti, Rodolfo; Asdente, Maria.

In: Stroke, Vol. 33, No. 3, 2002, p. 825-830.

Research output: Contribution to journalArticle

Guerrini, Uliano ; Sironi, Luigi ; Tremoli, Elena ; Cimino, Mauro ; Pollo, Bianca ; Calvio, Anna Maria ; Paoletti, Rodolfo ; Asdente, Maria. / New insights into brain damage in stroke-prone rats : A nuclear magnetic imaging study. In: Stroke. 2002 ; Vol. 33, No. 3. pp. 825-830.
@article{cc5e7972cb4b41fe845d16d51630cfb3,
title = "New insights into brain damage in stroke-prone rats: A nuclear magnetic imaging study",
abstract = "Background and Purpose - The spontaneously hypertensive stroke-prone rat (SHRSP) is an animal model for a complex form of cerebrovascular pathology. MRI provides an efficient and noninvasive tool for studying the time course of brain damage. The aim of this study was to gain new insights into the pathological phenomena responsible for the occurrence of brain injury in SHRSP with the use of the apparent diffusion coefficient of water (ADC), one of the most efficient MRI parameters for detecting brain abnormalities. To this end, the pattern of ADC variation observed in SHRSP was compared with that of focal ischemia induced in both SHRSP and Sprague-Dawley rats. Methods - Four groups of animals were studied: SHRSP developing spontaneous brain lesions fed with a salt-loaded (n=15, group 1) or standard diet (n=3, group 2) and Sprague-Dawley rats (n=8, group 3) and SHRSP (n=8, group 4) with permanent middle cerebral artery occlusion. ADC maps and T2-weighted images of brains were performed by MRI. After the rats were killed, the brains were removed and histologically processed. Results - There was no decrease in ADC during spontaneous stroke in the SHRSP fed with a normal or salt-enriched diet, while both the SHRSP and Sprague-Dawley rats with middle cerebral artery occlusion showed a marked decrease that lasted for 24 to 48 hours. Conclusions - Cerebral ischemia cannot be considered a major factor in the onset of spontaneous brain lesions in SHRSP, which show only vasogenic edema after the beginning of the damage with no evidence of metabolic impairment.",
keywords = "Animal models, Brain injuries, Diffusion, Magnetic resonance imaging, Middle cerebral artery occlusion, Rats",
author = "Uliano Guerrini and Luigi Sironi and Elena Tremoli and Mauro Cimino and Bianca Pollo and Calvio, {Anna Maria} and Rodolfo Paoletti and Maria Asdente",
year = "2002",
doi = "10.1161/hs0302.104111",
language = "English",
volume = "33",
pages = "825--830",
journal = "Stroke",
issn = "0039-2499",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - New insights into brain damage in stroke-prone rats

T2 - A nuclear magnetic imaging study

AU - Guerrini, Uliano

AU - Sironi, Luigi

AU - Tremoli, Elena

AU - Cimino, Mauro

AU - Pollo, Bianca

AU - Calvio, Anna Maria

AU - Paoletti, Rodolfo

AU - Asdente, Maria

PY - 2002

Y1 - 2002

N2 - Background and Purpose - The spontaneously hypertensive stroke-prone rat (SHRSP) is an animal model for a complex form of cerebrovascular pathology. MRI provides an efficient and noninvasive tool for studying the time course of brain damage. The aim of this study was to gain new insights into the pathological phenomena responsible for the occurrence of brain injury in SHRSP with the use of the apparent diffusion coefficient of water (ADC), one of the most efficient MRI parameters for detecting brain abnormalities. To this end, the pattern of ADC variation observed in SHRSP was compared with that of focal ischemia induced in both SHRSP and Sprague-Dawley rats. Methods - Four groups of animals were studied: SHRSP developing spontaneous brain lesions fed with a salt-loaded (n=15, group 1) or standard diet (n=3, group 2) and Sprague-Dawley rats (n=8, group 3) and SHRSP (n=8, group 4) with permanent middle cerebral artery occlusion. ADC maps and T2-weighted images of brains were performed by MRI. After the rats were killed, the brains were removed and histologically processed. Results - There was no decrease in ADC during spontaneous stroke in the SHRSP fed with a normal or salt-enriched diet, while both the SHRSP and Sprague-Dawley rats with middle cerebral artery occlusion showed a marked decrease that lasted for 24 to 48 hours. Conclusions - Cerebral ischemia cannot be considered a major factor in the onset of spontaneous brain lesions in SHRSP, which show only vasogenic edema after the beginning of the damage with no evidence of metabolic impairment.

AB - Background and Purpose - The spontaneously hypertensive stroke-prone rat (SHRSP) is an animal model for a complex form of cerebrovascular pathology. MRI provides an efficient and noninvasive tool for studying the time course of brain damage. The aim of this study was to gain new insights into the pathological phenomena responsible for the occurrence of brain injury in SHRSP with the use of the apparent diffusion coefficient of water (ADC), one of the most efficient MRI parameters for detecting brain abnormalities. To this end, the pattern of ADC variation observed in SHRSP was compared with that of focal ischemia induced in both SHRSP and Sprague-Dawley rats. Methods - Four groups of animals were studied: SHRSP developing spontaneous brain lesions fed with a salt-loaded (n=15, group 1) or standard diet (n=3, group 2) and Sprague-Dawley rats (n=8, group 3) and SHRSP (n=8, group 4) with permanent middle cerebral artery occlusion. ADC maps and T2-weighted images of brains were performed by MRI. After the rats were killed, the brains were removed and histologically processed. Results - There was no decrease in ADC during spontaneous stroke in the SHRSP fed with a normal or salt-enriched diet, while both the SHRSP and Sprague-Dawley rats with middle cerebral artery occlusion showed a marked decrease that lasted for 24 to 48 hours. Conclusions - Cerebral ischemia cannot be considered a major factor in the onset of spontaneous brain lesions in SHRSP, which show only vasogenic edema after the beginning of the damage with no evidence of metabolic impairment.

KW - Animal models

KW - Brain injuries

KW - Diffusion

KW - Magnetic resonance imaging

KW - Middle cerebral artery occlusion

KW - Rats

UR - http://www.scopus.com/inward/record.url?scp=0036192442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036192442&partnerID=8YFLogxK

U2 - 10.1161/hs0302.104111

DO - 10.1161/hs0302.104111

M3 - Article

C2 - 11872910

AN - SCOPUS:0036192442

VL - 33

SP - 825

EP - 830

JO - Stroke

JF - Stroke

SN - 0039-2499

IS - 3

ER -