New insights into cooperative binding of homeodomain transcription factors PREP1 and PBX1 to DNA

Chiara Zucchelli, E Ferrari, F Blasi, G Musco, C Bruckmann

Research output: Contribution to journalArticle

Abstract

PREP1 and PBX1 are homeodomain (HD) transcription factors that play crucial roles in embryonic development. Here, we present the first biophysical characterization of a PREP1 HD, and the NMR spectroscopic study of its DNA binding pocket. The data show that residues flanking the HD participate in DNA binding. The kinetic parameters for DNA binding of individual PREP1 and PBX1 HDs, and of their combination, show that isolated PREP1 and PBX1 HDs bind to DNA in a cooperative manner. A novel PREP1 motif, flanking the HD at the C-Terminus, is required for cooperativity.
Original languageEnglish
Article number40665
JournalScientific Reports
Volume7
Issue number3
DOIs
Publication statusPublished - 2017

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