New insights into the steen solution properties: Breakthrough in antioxidant effects via NOX2 downregulation

Roberto Carnevale, Giuseppe Biondi-Zoccai, Mariangela Peruzzi, Elena De Falco, Isotta Chimenti, Federico Venuta, Marco Anile, Daniele Diso, Elena Cavarretta, Antonino G M Marullo, Patrizio Sartini, Pasquale Pignatelli, Francesco Violi, Giacomo Frati

Research output: Contribution to journalArticle

Abstract

Ex vivo lung perfusion (EVLP) allows perfusion and reconditioning of retrieved lungs for organ transplantation. The Steen solution is specifically designed for this procedure but the mechanism through which it elicits its activity is still to be fully clarified. We speculated that Steen solution may encompass antioxidant properties allowing a reestablishment of pulmonary tissue homeostasis. Blood samples from 10 healthy volunteers were recruited. Platelets and white cells were incubated with Steen solution or buffer solution as control and stimulated with suitable agonists. Reactive oxidant species (ROS), soluble NOX2 (sNOX2-derived peptide), a marker of NADPH oxidase activation, p 47 phox translocation to cell membrane and isoprostanes production, as marker of oxidative stress, and nitric oxide (NO), a powerful vasodilator and antioxidant molecule, were measured upon cell stimulation. The Steen solution significantly inhibited p 47 phox translocation and NOX2 activation in platelets and white cells. Consistent with this finding was the reduction of oxidative stress as documented by a significantly lowered formation of ROS and isoprostanes by both platelets and white cells. Finally, cell incubation with Steen solution resulted in enhanced generation of NO. Herewith, we provide the first evidence that Steen solution possesses antioxidant properties via downregulation of NADPH oxidase activity and enhanced production of NO.

Original languageEnglish
Article number242180
JournalOxidative Medicine and Cellular Longevity
Volume2014
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry
  • Medicine(all)

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    Carnevale, R., Biondi-Zoccai, G., Peruzzi, M., De Falco, E., Chimenti, I., Venuta, F., Anile, M., Diso, D., Cavarretta, E., Marullo, A. G. M., Sartini, P., Pignatelli, P., Violi, F., & Frati, G. (2014). New insights into the steen solution properties: Breakthrough in antioxidant effects via NOX2 downregulation. Oxidative Medicine and Cellular Longevity, 2014, [242180]. https://doi.org/10.1155/2014/242180