New method based on capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) to monitor interaction between nanoparticles and the amyloid-β peptide

Davide Brambilla, Romain Verpillot, Myriam Taverna, Line De Kimpe, Benjamin Le Droumaguet, Julien Nicolas, Mara Canovi, Marco Gobbi, Francesco Mantegazza, Mario Salmona, Valérie Nicolas, Wiep Scheper, Patrick Couvreur, Karine Andrieux

Research output: Contribution to journalArticlepeer-review

Abstract

A novel application of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was proposed to efficiently detect and monitor the interaction between polymeric nanoparticles and the β-Amyloid peptide (Aβ1-42), a biomarker for Alzheimer's Disease (AD), at concentrations close to physiological conditions. The CE-LIF method allowed the interaction between PEGylated poly(alkyl cyanoacrylate) nanoparticles (NPs) and the soluble Aβ1-42 peptide monomers to be highlighted. These results were confirmed by surface plasmon resonance (SPR) and confocal laser scanning microscopy (CLSM). Whereas SPR showed an interaction between the NPs and the Aβ1-42 peptide, CLSM allowed the formation of large aggregates/assemblies at high NP and peptide concentrations to be visualized. All these results suggested that these nanoparticles could bind the Aβ1-42 peptide and influence its aggregation kinetics. Interestingly, the non-PEGylated poly(alkyl cyanoacrylate) NPs did not alter the aggregation kinetics of the Aβ1-42 peptide, thus emphasizing the high level of discrimination of the CE-LIF method with respect to NPs.

Original languageEnglish
Pages (from-to)10083-10089
Number of pages7
JournalAnalytical Chemistry
Volume82
Issue number24
DOIs
Publication statusPublished - Dec 15 2010

ASJC Scopus subject areas

  • Analytical Chemistry

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