New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage

Anita Ferraretto, Claudia Gravaghi, Elena Donetti, Stefania Cosentino, Bianca M. Donida, Marzia Bedoni, Giovanni Lombardi, Amelia Fiorilli, Guido Tettamanti

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.

Original languageEnglish
Pages (from-to)3919-3925
Number of pages7
JournalAnticancer Research
Volume27
Issue number6 B
Publication statusPublished - Nov 2007

Fingerprint

Caco-2 Cells
Phenotype
Microvilli
Population
Culture Media
Colon
Biomarkers
Cell Proliferation
Carcinoma
Cell Line
Pharmaceutical Preparations
Neoplasms

Keywords

  • Caco-2 cells
  • Differentiation
  • Intestine
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ferraretto, A., Gravaghi, C., Donetti, E., Cosentino, S., Donida, B. M., Bedoni, M., ... Tettamanti, G. (2007). New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage. Anticancer Research, 27(6 B), 3919-3925.

New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage. / Ferraretto, Anita; Gravaghi, Claudia; Donetti, Elena; Cosentino, Stefania; Donida, Bianca M.; Bedoni, Marzia; Lombardi, Giovanni; Fiorilli, Amelia; Tettamanti, Guido.

In: Anticancer Research, Vol. 27, No. 6 B, 11.2007, p. 3919-3925.

Research output: Contribution to journalArticle

Ferraretto, A, Gravaghi, C, Donetti, E, Cosentino, S, Donida, BM, Bedoni, M, Lombardi, G, Fiorilli, A & Tettamanti, G 2007, 'New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage', Anticancer Research, vol. 27, no. 6 B, pp. 3919-3925.
Ferraretto A, Gravaghi C, Donetti E, Cosentino S, Donida BM, Bedoni M et al. New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage. Anticancer Research. 2007 Nov;27(6 B):3919-3925.
Ferraretto, Anita ; Gravaghi, Claudia ; Donetti, Elena ; Cosentino, Stefania ; Donida, Bianca M. ; Bedoni, Marzia ; Lombardi, Giovanni ; Fiorilli, Amelia ; Tettamanti, Guido. / New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage. In: Anticancer Research. 2007 ; Vol. 27, No. 6 B. pp. 3919-3925.
@article{36c807c99064427787addcffc281ced9,
title = "New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage",
abstract = "Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.",
keywords = "Caco-2 cells, Differentiation, Intestine, Proliferation",
author = "Anita Ferraretto and Claudia Gravaghi and Elena Donetti and Stefania Cosentino and Donida, {Bianca M.} and Marzia Bedoni and Giovanni Lombardi and Amelia Fiorilli and Guido Tettamanti",
year = "2007",
month = "11",
language = "English",
volume = "27",
pages = "3919--3925",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "6 B",

}

TY - JOUR

T1 - New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage

AU - Ferraretto, Anita

AU - Gravaghi, Claudia

AU - Donetti, Elena

AU - Cosentino, Stefania

AU - Donida, Bianca M.

AU - Bedoni, Marzia

AU - Lombardi, Giovanni

AU - Fiorilli, Amelia

AU - Tettamanti, Guido

PY - 2007/11

Y1 - 2007/11

N2 - Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.

AB - Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.

KW - Caco-2 cells

KW - Differentiation

KW - Intestine

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=37549033814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37549033814&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 3919

EP - 3925

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 6 B

ER -