Abstract
Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.
| Original language | English |
|---|---|
| Pages (from-to) | 3919-3925 |
| Number of pages | 7 |
| Journal | Anticancer Research |
| Volume | 27 |
| Issue number | 6 B |
| Publication status | Published - Nov 2007 |
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Keywords
- Caco-2 cells
- Differentiation
- Intestine
- Proliferation
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage. / Ferraretto, Anita; Gravaghi, Claudia; Donetti, Elena; Cosentino, Stefania; Donida, Bianca M.; Bedoni, Marzia; Lombardi, Giovanni; Fiorilli, Amelia; Tettamanti, Guido.
In: Anticancer Research, Vol. 27, No. 6 B, 11.2007, p. 3919-3925.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage
AU - Ferraretto, Anita
AU - Gravaghi, Claudia
AU - Donetti, Elena
AU - Cosentino, Stefania
AU - Donida, Bianca M.
AU - Bedoni, Marzia
AU - Lombardi, Giovanni
AU - Fiorilli, Amelia
AU - Tettamanti, Guido
PY - 2007/11
Y1 - 2007/11
N2 - Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.
AB - Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.
KW - Caco-2 cells
KW - Differentiation
KW - Intestine
KW - Proliferation
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UR - http://www.scopus.com/inward/citedby.url?scp=37549033814&partnerID=8YFLogxK
M3 - Article
VL - 27
SP - 3919
EP - 3925
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 6 B
ER -