New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage

Anita Ferraretto, Claudia Gravaghi, Elena Donetti, Stefania Cosentino, Bianca M. Donida, Marzia Bedoni, Giovanni Lombardi, Amelia Fiorilli, Guido Tettamanti

Research output: Contribution to journalArticle

Abstract

Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.

Original languageEnglish
Pages (from-to)3919-3925
Number of pages7
JournalAnticancer Research
Volume27
Issue number6 B
Publication statusPublished - Nov 2007

Keywords

  • Caco-2 cells
  • Differentiation
  • Intestine
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Ferraretto, A., Gravaghi, C., Donetti, E., Cosentino, S., Donida, B. M., Bedoni, M., Lombardi, G., Fiorilli, A., & Tettamanti, G. (2007). New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage. Anticancer Research, 27(6 B), 3919-3925.