Sviluppo di nuove metodiche in vitro per la diagnosi di dermatite allergica da contatto al nichel

Translated title of the contribution: New methods for the diagnosis in vitro of allergic contact dermatitis to nickel

Chiara Ottaviani, Francesca Nasorri, Alessandra Frezzolini, Giampiero Girolomoni, Ornella De Pità, Andrea Cavani

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the work is to identify new in vitro approaches for the diagnosis of allergic contact dermatitis (ACD) to nickel, based on the functional characterization of peripheral blood-derived T cell subsets. Peripheral blood mononuclear cells were isolated from nickel allergic individuals and healthy controls and purified with immunomagnetic beads to obtain CD4+ and CD8+ fractions. Pure T cell populations were examined for nickel responsiveness and expression of activation markers by cytofluorimetric analysis in the presence of 20μg/ml nickel sulphate and autologous antigen presenting cells. Results demonstrate that 41 out of 45 nickel allergic, but only 3 out of 45 healthy donors displayed a significant (stimulation index ≥ 3) CD8+ T cell response to nickel, thus suggesting a high correlation with ACD expression to the metal. In addition, nickel exposure induced the activation marker CD69 in CD8+ T cells from allergic, but not non-allergic subjects. In contrast, CD4+ T cell fraction from both groups of donors proliferated to nickel in vitro and displayed membrane expression of CD69. Our findings indicate that the magnitude of CD8+, but not CD4+ T cell responses to nickel can be used as a marker for the diagnosis of ACD to the metal.

Translated title of the contributionNew methods for the diagnosis in vitro of allergic contact dermatitis to nickel
Original languageItalian
Pages (from-to)88-94
Number of pages7
JournalAnnali Italiani di Dermatologia Clinica e Sperimentale
Volume57
Issue number2-3
Publication statusPublished - May 2003

ASJC Scopus subject areas

  • Dermatology

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