The aim of the work is to identify new in vitro approaches for the diagnosis of allergic contact dermatitis (ACD) to nickel, based on the functional characterization of peripheral blood-derived T cell subsets. Peripheral blood mononuclear cells were isolated from nickel allergic individuals and healthy controls and purified with immunomagnetic beads to obtain CD4+ and CD8+ fractions. Pure T cell populations were examined for nickel responsiveness and expression of activation markers by cytofluorimetric analysis in the presence of 20μg/ml nickel sulphate and autologous antigen presenting cells. Results demonstrate that 41 out of 45 nickel allergic, but only 3 out of 45 healthy donors displayed a significant (stimulation index ≥ 3) CD8+ T cell response to nickel, thus suggesting a high correlation with ACD expression to the metal. In addition, nickel exposure induced the activation marker CD69 in CD8+ T cells from allergic, but not non-allergic subjects. In contrast, CD4+ T cell fraction from both groups of donors proliferated to nickel in vitro and displayed membrane expression of CD69. Our findings indicate that the magnitude of CD8+, but not CD4+ T cell responses to nickel can be used as a marker for the diagnosis of ACD to the metal.
|Translated title of the contribution||New methods for the diagnosis in vitro of allergic contact dermatitis to nickel|
|Number of pages||7|
|Journal||Annali Italiani di Dermatologia Clinica e Sperimentale|
|Publication status||Published - May 2003|
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