New model for bone resorption study in vitro: Human osteoclast-like cells from giant cell tumors of bone

Maria Grano, Silvia Colucci, Michele De Bellis, Paola Zigrino, Luigi Argentino, Giovanni Zambonin, Massimo Serra, Katia Scotlandi, Anna Teti, Alberta Zambonin Zallone

Research output: Contribution to journalArticle

Abstract

Cells harvested from 12 human giant cell tumors of bone and kept in culture for several passages were characterized for bone-resorbing capability, total and tartrate-resistant acid phosphatase activity, response to the calciotropic hormone calcitonin, cell proliferation, multinucleation after passages, and presence of calcium sensing. Cells obtained from three tumors presented a complete panel of osteoclast characteristics and maintained their multinuclearity after several passages. Cells from four other tumors increased their cAMP levels after treatment with calcitonin, and the other five apparently consisted of cells of stromal origin. These human cell populations with osteoclast characteristics may provide valid in vitro models for the investigation of osteoclastic differentiation and activity.

Original languageEnglish
Pages (from-to)1013-1020
Number of pages8
JournalJournal of Bone and Mineral Research
Volume9
Issue number7
Publication statusPublished - Jul 1994

ASJC Scopus subject areas

  • Surgery

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    Grano, M., Colucci, S., De Bellis, M., Zigrino, P., Argentino, L., Zambonin, G., Serra, M., Scotlandi, K., Teti, A., & Zallone, A. Z. (1994). New model for bone resorption study in vitro: Human osteoclast-like cells from giant cell tumors of bone. Journal of Bone and Mineral Research, 9(7), 1013-1020.