New Portland Cement-based Materials for Endodontics Mixed with Articaine Solution: A Study of Cellular Response

Maria Giovanna Gandolfi, Francesca Perut, Gabriela Ciapetti, Romano Mongiorgi, Carlo Prati

Research output: Contribution to journalArticle

Abstract

The biocompatibility of innovative tetrasilicate cements proposed for root-end filling restorations was tested. White ProRoot-MTA and AH Plus were used as control. The new cements were mixed with a local anesthetic solution (4% articaine) to form a paste. Human osteoblast-like cells Saos-2 were challenged in short-term cultures (72 hours) with solid materials and with material extracts prepared in culture medium. Cell growth and viability, cellular attachment, and morphologic features were assessed to verify cell/material interactions. No acute toxicity was exerted by the experimental cements in the assay systems. On solid samples Saos-2 adhered and proliferated on all the experimental cements and on MTA. The ultrastructural findings revealed that Saos-2 were able to adhere and to spread. The maintenance of the osteoblastic phenotype on the innovative cements was confirmed by the alkaline phosphatase assay. All experimental cements prepared with articaine supported the growth of bone-like cells, showing suitable properties to be used as canal sealers and root-end filling materials.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalJournal of Endodontics
Volume34
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Biocompatibility
  • calcium silicate
  • crystals
  • cytotoxicity
  • endodontic materials
  • fluoride
  • phyllosilicate
  • Portland cement

ASJC Scopus subject areas

  • Dentistry(all)

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