Nuova possibilitá di caratterizzazione genetica dell'Human Papillomavirus nella patologia dell'apparato genitale maschile e femminile.

Translated title of the contribution: New possibility of genetic characterization of human papillomavirus in pathology of male and female genitalia

F. Negri, F. Montale, M. Pollastro, C. Savioli, F. Comanducci, A. Morando

Research output: Contribution to journalArticle

Abstract

Several epidemiologic studies point to the role of different sublines of Human Papillomavirus (HPV) in the development of neoplasias of the genito-rectal tract. The spread of the virus in the population makes it necessary to constantly investigate this pathogen in order to adopt a correct therapeutic approach and follow the evolution of associated disease. This paper gives an epidemiologic outline of HPV infection in the adult population--males and females--based on a correlated study of the relationship between the lesion and the presence of the virus and its sublines. The methods used both traditional examinations (colposcopy, cytology, histology) and innovative chemiluminescence molecular biology techniques. Chemiluminescence molecular hybridization includes the use of RNA probes which, on the basis the identification of different viral strains, allow a subdivision of infections into low and high/medium risk. The test resulted positive in 60.7% of the cases in which there was clinical and cyto-histopathological evidence of HPV infection. Notwithstanding the high percentage of negative results in the cases of condylomatous disease, the method used has proved to be easily applicable and more sensitive than other molecular biology techniques (comparison by in situ hybridization and Southern blot analysis). Moreover, from the clinical point of view, it seems to provide useful data for a correct diagnosis and monitoring of patients.

Original languageItalian
Pages (from-to)377-383
Number of pages7
JournalMinerva Ginecologica
Volume46
Issue number7-8
Publication statusPublished - Jul 1994

Fingerprint

Male Genitalia
Female Genitalia
Medical Genetics
Papillomavirus Infections
Pathology
Luminescence
Molecular Biology
Viruses
RNA Probes
Colposcopy
Physiologic Monitoring
Southern Blotting
Population
In Situ Hybridization
Cell Biology
Epidemiologic Studies
Histology
Infection
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Negri, F., Montale, F., Pollastro, M., Savioli, C., Comanducci, F., & Morando, A. (1994). Nuova possibilitá di caratterizzazione genetica dell'Human Papillomavirus nella patologia dell'apparato genitale maschile e femminile. Minerva Ginecologica, 46(7-8), 377-383.

Nuova possibilitá di caratterizzazione genetica dell'Human Papillomavirus nella patologia dell'apparato genitale maschile e femminile. / Negri, F.; Montale, F.; Pollastro, M.; Savioli, C.; Comanducci, F.; Morando, A.

In: Minerva Ginecologica, Vol. 46, No. 7-8, 07.1994, p. 377-383.

Research output: Contribution to journalArticle

Negri, F, Montale, F, Pollastro, M, Savioli, C, Comanducci, F & Morando, A 1994, 'Nuova possibilitá di caratterizzazione genetica dell'Human Papillomavirus nella patologia dell'apparato genitale maschile e femminile.', Minerva Ginecologica, vol. 46, no. 7-8, pp. 377-383.
Negri, F. ; Montale, F. ; Pollastro, M. ; Savioli, C. ; Comanducci, F. ; Morando, A. / Nuova possibilitá di caratterizzazione genetica dell'Human Papillomavirus nella patologia dell'apparato genitale maschile e femminile. In: Minerva Ginecologica. 1994 ; Vol. 46, No. 7-8. pp. 377-383.
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