New rapid staining methods of Cryptosporidium oocysts in stools.

G. Chichino, A. Bruno, C. Cevini, C. Atzori, S. Gatti, M. Scaglia

Research output: Contribution to journalArticlepeer-review


Two new extemporaneous negative-staining methods are proposed to detect Cryptosporidium oocysts in stools, using light-green and merbromine in 1% and 2% aqueous solution, respectively. A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. We propose their use in routine diagnosis for enteric cryptosporidiosis.

Original languageEnglish
JournalJournal of Protozoology
Issue number6
Publication statusPublished - Nov 1991

ASJC Scopus subject areas

  • Parasitology


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