New ribosome-inactivating proteins with polynucleotide: adenosine glycosidase and antiviral activities from Basella rubra L. and Bougainvillea spectabilis Willd

Andrea Bolognesi, Letizia Polito, Fabiola Olivieri, Paola Valbonesi, Luigi Barbieri, M. Giulia Bartelli, M. Vittoria Carusi, Eugenio Benvenuto, Francesca Del Vecchio Blanco, Antimo Di Maro, Augusto Parente, Mario Di Loreto, Fiorenzo Stirpe

Research output: Contribution to journalArticle

Abstract

New single-chain (type 1) ribosome-inactivating proteins (RIPs) were isolated from the seeds of Basella rubra L. (two proteins) and from the leaves of Bougainvillea spectabilis Willd. (one protein). These RIPs inhibit protein synthesis both in a cell-free system, with an IC50 (concentration causing 50% inhibition) in the 10-10 M range, and by various cell lines, with IC50s in the 10-8-10-6 M range. All three RIPs released adenine not only from rat liver ribosomes but also from Escherichia coli rRNA, polyadenylic acid, herring sperm DNA, and artichoke mottled crinkle virus (AMCV) genomic RNA, thus being polynucleotide:adenosine glycosidases. The proteins from Basella rubra had toxicity to mice similar to that of most type I RIPs (Barbieri et al., 1993, Biochim Biophys Acta 1154: 237-282) with an LD50 (concentration that is 50% lethal) ≤8 mg·kg-1 body weight, whilst the RIP from Bougainvillea spectabilis had an LD50 >32 mg·kg-1. The N-terminal sequence of the two RIPs from Basella rubra had 80-93% identity, whereas it differed from the sequence of the RIP from Bougainvillea spectabilis. When tested with antibodies against various RIPs, the RIPs from Basella gave some cross-reactivity with sera against dianthin 32, and weak cross-reactivity with momordin I and momorcochin-S, whilst the RIP from Bougainvillea did not cross-react with any antiserum tested. An RIP from Basella rubra and one from Bougainvillea spectabilis were tested for antiviral activity, and both inhibited infection of Nicotiana benthamiana by AMCV.

Original languageEnglish
Pages (from-to)422-429
Number of pages8
JournalPlanta
Volume203
Issue number4
DOIs
Publication statusPublished - Dec 1997

Fingerprint

Nyctaginaceae
Basella alba
Bougainvillea
Ribosome Inactivating Proteins
polynucleotides
glycosidases
adenosine
ribosomes
Antiviral Agents
proteins
Cynara scolymus
Artichoke mottled crinkle virus
Lethal Dose 50
Type 1 Ribosome Inactivating Proteins
Proteins
lethal dose 50
cross reaction
Basella
Poly A
Cell-Free System

Keywords

  • Antiviral protein
  • Basella
  • Bougainvillea
  • Polynucleotide:adenosine glycosidase
  • Ribosome-inactivating protein

ASJC Scopus subject areas

  • Plant Science

Cite this

New ribosome-inactivating proteins with polynucleotide : adenosine glycosidase and antiviral activities from Basella rubra L. and Bougainvillea spectabilis Willd. / Bolognesi, Andrea; Polito, Letizia; Olivieri, Fabiola; Valbonesi, Paola; Barbieri, Luigi; Giulia Bartelli, M.; Vittoria Carusi, M.; Benvenuto, Eugenio; Del Vecchio Blanco, Francesca; Di Maro, Antimo; Parente, Augusto; Di Loreto, Mario; Stirpe, Fiorenzo.

In: Planta, Vol. 203, No. 4, 12.1997, p. 422-429.

Research output: Contribution to journalArticle

Bolognesi, A, Polito, L, Olivieri, F, Valbonesi, P, Barbieri, L, Giulia Bartelli, M, Vittoria Carusi, M, Benvenuto, E, Del Vecchio Blanco, F, Di Maro, A, Parente, A, Di Loreto, M & Stirpe, F 1997, 'New ribosome-inactivating proteins with polynucleotide: adenosine glycosidase and antiviral activities from Basella rubra L. and Bougainvillea spectabilis Willd', Planta, vol. 203, no. 4, pp. 422-429. https://doi.org/10.1007/s004250050209
Bolognesi, Andrea ; Polito, Letizia ; Olivieri, Fabiola ; Valbonesi, Paola ; Barbieri, Luigi ; Giulia Bartelli, M. ; Vittoria Carusi, M. ; Benvenuto, Eugenio ; Del Vecchio Blanco, Francesca ; Di Maro, Antimo ; Parente, Augusto ; Di Loreto, Mario ; Stirpe, Fiorenzo. / New ribosome-inactivating proteins with polynucleotide : adenosine glycosidase and antiviral activities from Basella rubra L. and Bougainvillea spectabilis Willd. In: Planta. 1997 ; Vol. 203, No. 4. pp. 422-429.
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abstract = "New single-chain (type 1) ribosome-inactivating proteins (RIPs) were isolated from the seeds of Basella rubra L. (two proteins) and from the leaves of Bougainvillea spectabilis Willd. (one protein). These RIPs inhibit protein synthesis both in a cell-free system, with an IC50 (concentration causing 50{\%} inhibition) in the 10-10 M range, and by various cell lines, with IC50s in the 10-8-10-6 M range. All three RIPs released adenine not only from rat liver ribosomes but also from Escherichia coli rRNA, polyadenylic acid, herring sperm DNA, and artichoke mottled crinkle virus (AMCV) genomic RNA, thus being polynucleotide:adenosine glycosidases. The proteins from Basella rubra had toxicity to mice similar to that of most type I RIPs (Barbieri et al., 1993, Biochim Biophys Acta 1154: 237-282) with an LD50 (concentration that is 50{\%} lethal) ≤8 mg·kg-1 body weight, whilst the RIP from Bougainvillea spectabilis had an LD50 >32 mg·kg-1. The N-terminal sequence of the two RIPs from Basella rubra had 80-93{\%} identity, whereas it differed from the sequence of the RIP from Bougainvillea spectabilis. When tested with antibodies against various RIPs, the RIPs from Basella gave some cross-reactivity with sera against dianthin 32, and weak cross-reactivity with momordin I and momorcochin-S, whilst the RIP from Bougainvillea did not cross-react with any antiserum tested. An RIP from Basella rubra and one from Bougainvillea spectabilis were tested for antiviral activity, and both inhibited infection of Nicotiana benthamiana by AMCV.",
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AU - Del Vecchio Blanco, Francesca

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