New trends in diagnosis and control strategies for implant infections

Carla Renata Arciola, Lucio Montanaro, John William Costerton

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In implant infections, a quick and reliable identification of the etiological agent is crucial to realizing efficacious therapies. Among molecular methods, automated ribotyping has proven to be an accurate and rapid technique. More recently, MALDI-TOF/MS and PCR-electrospray ionization (ESI)/ MS have been applied successfully to microbiological diagnosis. In implant infections, biofilm is still the major problem for bacterial persistence and recalcitrance to antibiotic therapy. Among biofilmdisrupting agents, enzymes promise the greatest therapeutic possibilities. DNase I degrades biofilm extracellular DNA and has been shown to sensitize biofilm to various biocides and anionic detergents, while dispersin B acts on biofilm exopolysaccharide and, combined with antiseptic, gives a broad-spectrum antibiofilm and antimicrobial activity. The novel antimicrobial approach based on photodynamic treatment (PDT) applies, in combination with antibiotics, to the implant or medical devices reachable by optical fibers. Better progress could be gained by the development of infection- resistant biomaterials able to both inhibit bacterial adhesion and promote tissue integration. New knowledge regarding the fibronectin-mediated internalization of Staphylococcus aureus by osteoblasts, and on its role in the pathogenesis of implant-related osteomyelitis, paves the way for the development of vaccines against staphylococcal adhesins, to prevent both adhesion on biomaterials and bacterial invasion of bone cells.

Original languageEnglish
Pages (from-to)727-736
Number of pages10
JournalInternational Journal of Artificial Organs
Volume34
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Biofilms
Biocompatible Materials
Antibiotics
Infection
Biomaterials
Staphylococcal Vaccines
Adhesion
Bacterial Adhesion
Anti-Bacterial Agents
Ribotyping
Optical Fibers
Electrospray ionization
Biocides
Local Anti-Infective Agents
Vaccines
Disinfectants
Deoxyribonuclease I
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Osteoblasts
Detergents

Keywords

  • Antibiofilm agents
  • Biofilm
  • Extracellular DNA
  • Implant infections
  • Infection resistant materials
  • Microbiological diagnosis

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

New trends in diagnosis and control strategies for implant infections. / Arciola, Carla Renata; Montanaro, Lucio; Costerton, John William.

In: International Journal of Artificial Organs, Vol. 34, No. 9, 09.2011, p. 727-736.

Research output: Contribution to journalArticle

Arciola, Carla Renata ; Montanaro, Lucio ; Costerton, John William. / New trends in diagnosis and control strategies for implant infections. In: International Journal of Artificial Organs. 2011 ; Vol. 34, No. 9. pp. 727-736.
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