Newborn genome-wide DNA methylation in association with pregnancy anxiety reveals a potential role for GABBR1

Elise Beau Vangeel, Ehsan Pishva, Titia Hompes, Daniel van den Hove, Diether Lambrechts, Karel Allegaert, Kathleen Freson, Benedetta Izzi, Stephan Claes

Research output: Contribution to journalArticlepeer-review


Background: There is increasing evidence for the role of prenatal stress in shaping offspring DNA methylation and disease susceptibility. In the current study, we aimed to identify genes and pathways associated with pregnancy anxiety using a genome-wide DNA methylation approach. Methods: We selected 22 versus 23 newborns from our Prenatal Early Life Stress (PELS) cohort, exposed to the lowest or highest degree of maternal pregnancy anxiety, respectively. Cord blood genome-wide DNA methylation was assayed using the HumanMethylation450 BeadChip (HM450, n=45) and candidate gene methylation using EpiTYPER (n=80). Cortisol levels were measured at 2, 4, and 12months of age to test infant stress system (re)activity. Results: Data showed ten differentially methylated regions (DMR) when comparing newborns exposed to low versus high pregnancy anxiety scores. We validated a top DMR in the GABA-B receptor subunit 1 gene (GABBR1) revealing the association with pregnancy anxiety particularly in male newborns (most significant CpG Pearson R=0.517, p=0.002; average methylation Pearson R=0.332, p=0.039). Cord blood GABBR1 methylation was associated with infant cortisol levels in response to a routine vaccination at 4months old. Conclusions: In conclusion, our results show that pregnancy anxiety is associated with differential DNA methylation patterns in newborns and that our candidate gene GABBR1 is associated with infant hypothalamic-pituitary-adrenal axis response to a stressor. Our findings reveal a potential role for GABBR1 methylation in association with stress and provide grounds for further research.

Original languageEnglish
Article number107
JournalClinical Epigenetics
Issue number1
Publication statusPublished - Oct 3 2017


  • DNA methylation
  • GABBR1
  • Gender differences
  • HPA axis
  • Pregnancy anxiety
  • Prenatal stress

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology
  • Genetics(clinical)

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