Next-generation sequencing and DNA replication in human cells: the future has arrived

Research output: Contribution to journalArticlepeer-review


Accurate regulation of DNA replication ensures faithful transmission of eukaryotic genomes and maintenance of genomic stability and chromatin organization. However, by itself the replication process is a threat for both DNA and chromatin integrity. This becomes particularly relevant in cancer cells, where activated oncogenes induce replication-stress, including unscheduled initiation, fork stalling and collapse and, ultimately, genomic instability. Studies addressing the relationship between (epi)genome integrity and disease have been hampered by our poor knowledge of the mechanisms regulating where and when eukaryotic replication initiates. Recently developed genome-scale methods for the analysis of DNA replication in mammals will contribute to the identification of missing links between replication, chromatin regulation and genome stability in normal and cancer cells.

Original languageEnglish
Pages (from-to)683-693
Number of pages11
JournalFuture Oncology
Issue number4
Publication statusPublished - 2014


  • DNA replication
  • origin recognition complex
  • replication stress
  • replication timing
  • transcription start sites

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Medicine(all)


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