Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 489 |
| Number of pages | 11 |
| Journal | Front. Oncol. |
| Volume | 10 |
| DOIs | |
| Publication status | Published - 2020 |
Keywords
- anchored multiplex PCR
- fusion transcripts
- hybrid capture-based panel
- molecular diagnosis
- NGS
- sarcoma
- Article
- bioinformatics
- controlled study
- dermatofibrosarcoma protuberans
- fluorescence in situ hybridization
- gene expression
- gene sequence
- high throughput sequencing
- human
- human tissue
- major clinical study
- multiplex polymerase chain reaction
- neuroectoderm tumor
- real time polymerase chain reaction
- RNA extraction
- RNA sequence
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Next-Generation Sequencing Approaches for the Identification of Pathognomonic Fusion Transcripts in Sarcomas: The Experience of the Italian ACC Sarcoma Working Group : Frontiers in Oncology. / Racanelli, D.; Brenca, M.; Baldazzi, D.; Goeman, F.; Casini, B.; De Angelis, B.; Guercio, M.; Milano, G.M.; Tamborini, E.; Busico, A.; Dagrada, G.; Garofalo, C.; Caruso, C.; Brunello, A.; Pignochino, Y.; Berrino, E.; Grignani, G.; Scotlandi, K.; Parra, A.; Hattinger, C.M.; Ibrahim, T.; Mercatali, L.; De Vita, A.; Carriero, M.V.; Pallocca, M.; Loria, R.; Covello, R.; Sbaraglia, M.; Dei Tos, A.P.; Falcioni, R.; Maestro, R.
In: Front. Oncol., Vol. 10, 2020, p. 489.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Next-Generation Sequencing Approaches for the Identification of Pathognomonic Fusion Transcripts in Sarcomas: The Experience of the Italian ACC Sarcoma Working Group
T2 - Frontiers in Oncology
AU - Racanelli, D.
AU - Brenca, M.
AU - Baldazzi, D.
AU - Goeman, F.
AU - Casini, B.
AU - De Angelis, B.
AU - Guercio, M.
AU - Milano, G.M.
AU - Tamborini, E.
AU - Busico, A.
AU - Dagrada, G.
AU - Garofalo, C.
AU - Caruso, C.
AU - Brunello, A.
AU - Pignochino, Y.
AU - Berrino, E.
AU - Grignani, G.
AU - Scotlandi, K.
AU - Parra, A.
AU - Hattinger, C.M.
AU - Ibrahim, T.
AU - Mercatali, L.
AU - De Vita, A.
AU - Carriero, M.V.
AU - Pallocca, M.
AU - Loria, R.
AU - Covello, R.
AU - Sbaraglia, M.
AU - Dei Tos, A.P.
AU - Falcioni, R.
AU - Maestro, R.
N1 - Cited By :3 Export Date: 18 February 2021 Correspondence Address: Maestro, R.; Unit of Oncogenetics and Functional Oncogenomics, Italy; email: maestro@cro.it Funding details: American Ceramic Circle, ACC Funding text 1: For their suggestions and support, the authors are grateful to: Valentina Laquintana (Regina Elena National Cancer Institute, Rome); Sara Piccinin, Daniela Gasparotto, Kelly Fassetta, Beatrice Valenti (Centro di Riferimento Oncologico, CRO Aviano); Franco Locatelli, Simona Caruso, Ida Russo, Rita Alaggio, Rita De Vito, Emanuele Agolini, Martina Rinelli (Bambino Ges? Children's Hospital, IRCCS, Rome); Carolina Zamuner (Veneto Institute of Oncology, Padua, Italy); Massimo Serra, Laura Pazzaglia, Marco Gambarotti, Stefania Benini, Alberto Righi (Istituto Ortopedico Rizzoli, Bologna); Federica Pieri, Michela Tebaldi, Elisa Chiadini (Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, Meldola). Special thanks for their work go to secretaries, preclinical, and clinical coordinators of the ACC sarcoma working group, the Italian Sarcoma Group (ISG), the Rizzoli and the CRO Aviano Institutes. Funding. This work was supported by the Ministry of Health and Alleanza Contro il Cancro (ACC). 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Melsted, P., Hateley, S., Joseph, I.C., Pimentel, H., Bray, N., Pachter, L., Fusion detection and quantification by pseudoalignment (2017) bioRxiv, p. 166322; Lam, S.W., Cleton-Jansen, A.-M., Cleven, A.H.G., Ruano, D., van Wezel, T., Szuhai, K., Molecular analysis of gene fusions in bone and soft tissue tumors by anchored multiplex PCR-based targeted next-generation sequencing (2018) J Mol Diagn, 20, pp. 653-663. , 30139549; Pierron, G., Tirode, F., Lucchesi, C., Reynaud, S., Ballet, S., Cohen-Gogo, S., A new subtype of bone sarcoma defined by BCOR-CCNB3 gene fusion (2012) Nat Genet, 44, pp. 461-466. , 22387997; Panagopoulos, I., Thorsen, J., Gorunova, L., Haugom, L., Bjerkehagen, B., Davidson, B., Fusion of the ZC3H7B and BCOR genes in endometrial stromal sarcomas carrying an X;22-translocation (2013) Genes Chromosomes Cancer, 52, pp. 610-618. , 23580382; Specht, K., Zhang, L., Sung, Y.-S., Nucci, M., Dry, S., Vaiyapuri, S., Novel BCOR-MAML3 and ZC3H7B-BCOR Gene Fusions in Undifferentiated Small Blue Round Cell Sarcomas (2016) Am J Surg Pathol, 40, pp. 433-442. , 26752546; 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PY - 2020
Y1 - 2020
N2 - This work describes the set-up of a shared platform among the laboratories of the Alleanza Contro il Cancro (ACC) Italian Research Network for the identification of fusion transcripts in sarcomas by using Next Generation Sequencing (NGS). Different NGS approaches, including anchored multiplex PCR and hybrid capture-based panels, were employed to profile a large set of sarcomas of different histotypes. The analysis confirmed the reliability of NGS RNA-based approaches in detecting sarcoma-specific rearrangements. Overall, the anchored multiplex PCR assay proved to be a fast and easy-to-analyze approach for routine diagnostics laboratories. © Copyright © 2020 Racanelli, Brenca, Baldazzi, Goeman, Casini, De Angelis, Guercio, Milano, Tamborini, Busico, Dagrada, Garofalo, Caruso, Brunello, Pignochino, Berrino, Grignani, Scotlandi, Parra, Hattinger, Ibrahim, Mercatali, De Vita, Carriero, Pallocca, Loria, Covello, Sbaraglia, Dei Tos, Falcioni and Maestro.
AB - This work describes the set-up of a shared platform among the laboratories of the Alleanza Contro il Cancro (ACC) Italian Research Network for the identification of fusion transcripts in sarcomas by using Next Generation Sequencing (NGS). Different NGS approaches, including anchored multiplex PCR and hybrid capture-based panels, were employed to profile a large set of sarcomas of different histotypes. The analysis confirmed the reliability of NGS RNA-based approaches in detecting sarcoma-specific rearrangements. Overall, the anchored multiplex PCR assay proved to be a fast and easy-to-analyze approach for routine diagnostics laboratories. © Copyright © 2020 Racanelli, Brenca, Baldazzi, Goeman, Casini, De Angelis, Guercio, Milano, Tamborini, Busico, Dagrada, Garofalo, Caruso, Brunello, Pignochino, Berrino, Grignani, Scotlandi, Parra, Hattinger, Ibrahim, Mercatali, De Vita, Carriero, Pallocca, Loria, Covello, Sbaraglia, Dei Tos, Falcioni and Maestro.
KW - anchored multiplex PCR
KW - fusion transcripts
KW - hybrid capture-based panel
KW - molecular diagnosis
KW - NGS
KW - sarcoma
KW - Article
KW - bioinformatics
KW - controlled study
KW - dermatofibrosarcoma protuberans
KW - fluorescence in situ hybridization
KW - gene expression
KW - gene sequence
KW - high throughput sequencing
KW - human
KW - human tissue
KW - major clinical study
KW - multiplex polymerase chain reaction
KW - neuroectoderm tumor
KW - real time polymerase chain reaction
KW - RNA extraction
KW - RNA sequence
U2 - 10.3389/fonc.2020.00489
DO - 10.3389/fonc.2020.00489
M3 - Article
VL - 10
SP - 489
JO - Front. Oncol.
JF - Front. Oncol.
SN - 2234-943X
ER -