@article{6eed85d3731747f28f663a46dc3d228c,
title = "Next-Generation Sequencing Approaches for the Identification of Pathognomonic Fusion Transcripts in Sarcomas: The Experience of the Italian ACC Sarcoma Working Group",
abstract = "This work describes the set-up of a shared platform among the laboratories of the Alleanza Contro il Cancro (ACC) Italian Research Network for the identification of fusion transcripts in sarcomas by using Next Generation Sequencing (NGS). Different NGS approaches, including anchored multiplex PCR and hybrid capture-based panels, were employed to profile a large set of sarcomas of different histotypes. The analysis confirmed the reliability of NGS RNA-based approaches in detecting sarcoma-specific rearrangements. Overall, the anchored multiplex PCR assay proved to be a fast and easy-to-analyze approach for routine diagnostics laboratories.",
keywords = "anchored multiplex PCR, fusion transcripts, hybrid capture-based panel, molecular diagnosis, NGS, sarcoma",
author = "Dominga Racanelli and Monica Brenca and Davide Baldazzi and Frauke Goeman and Beatrice Casini and {De Angelis}, Biagio and Marika Guercio and Milano, {Giuseppe Maria} and Elena Tamborini and Adele Busico and Gianpaolo Dagrada and Cecilia Garofalo and Chiara Caruso and Antonella Brunello and Ymera Pignochino and Enrico Berrino and Giovanni Grignani and Katia Scotlandi and Alessandro Parra and Hattinger, {Claudia Maria} and Toni Ibrahim and Laura Mercatali and {De Vita}, Alessandro and Carriero, {Maria Vincenza} and Matteo Pallocca and Rossella Loria and Renato Covello and Marta Sbaraglia and {Dei Tos}, {Angelo Paolo} and Rita Falcioni and Roberta Maestro",
note = "M1 - 489",
year = "2020",
month = apr,
day = "15",
doi = "10.3389/fonc.2020.00489",
language = "English",
volume = "10",
pages = "1--11",
journal = "Frontiers in Oncology",
issn = "2234-943X",
publisher = "Frontiers Media S. A.",
}