This work describes the set-up of a shared platform among the laboratories of the Alleanza Contro il Cancro (ACC) Italian Research Network for the identification of fusion transcripts in sarcomas by using Next Generation Sequencing (NGS). Different NGS approaches, including anchored multiplex PCR and hybrid capture-based panels, were employed to profile a large set of sarcomas of different histotypes. The analysis confirmed the reliability of NGS RNA-based approaches in detecting sarcoma-specific rearrangements. Overall, the anchored multiplex PCR assay proved to be a fast and easy-to-analyze approach for routine diagnostics laboratories.
Original languageEnglish
Article number489
Pages (from-to)1-11
Number of pages11
JournalFrontiers in Oncology
Publication statusPublished - Apr 15 2020


  • anchored multiplex PCR
  • fusion transcripts
  • hybrid capture-based panel
  • molecular diagnosis
  • NGS
  • sarcoma


Dive into the research topics of 'Next-Generation Sequencing Approaches for the Identification of Pathognomonic Fusion Transcripts in Sarcomas: The Experience of the Italian ACC Sarcoma Working Group'. Together they form a unique fingerprint.

Cite this