NF-κB localization in multiple myeloma plasma cells and mesenchymal cells

Concetta Conticello, Raffaella Giuffrida, Luana Adamo, Gabriele Anastasi, Daniela Martinetti, Edvige Salomone, Cristina Colarossi, Gabriella Amato, Ausilia Gorgone, Alessandra Romano, Gioacchin Iannolo, Ruggero De Maria, Rosario Giustolisi, Massimo Gulisano, Francesco Di Raimondo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Several reports demonstrated that the activation of Nuclear Factor-kappa B NF-κB is essential for the pathogenesis of multiple myeloma (MM). We analyzed the nuclear localization of NF-κB in MM-cells derived from 60 different patients with MM at presentation and in relapse, as well as in three myeloma cell lines. Nuclear localization (the active form) of NF-κB was detected in only one MM-sample from a refractory patient and in two samples from relapsed patients, while all the other samples, including the MM-cell lines, almost exclusively express the cytoplasmic (inactive) form of NF-κB. In mesenchymal cells from MM-patients NF-κB was clearly present in the nucleus. In addition, the proteasome inhibitor Bortezomib, which is described to antagonize NF-κB activity, had a consistent antitumor activity against both chemoresistant and chemosensitive MM-cells, regardless the NF-κB localization, thus suggesting the existence of other molecular targets of proteasome inhibitors in MM.

Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalLeukemia Research
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Plasma Cells
Multiple Myeloma
Proteasome Inhibitors
Cell Line
NF-kappa B
Recurrence

Keywords

  • Bortezomib
  • Mesenchymal cells
  • Multiple myeloma
  • NF-κB

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Conticello, C., Giuffrida, R., Adamo, L., Anastasi, G., Martinetti, D., Salomone, E., ... Di Raimondo, F. (2011). NF-κB localization in multiple myeloma plasma cells and mesenchymal cells. Leukemia Research, 35(1), 52-60. https://doi.org/10.1016/j.leukres.2010.06.023

NF-κB localization in multiple myeloma plasma cells and mesenchymal cells. / Conticello, Concetta; Giuffrida, Raffaella; Adamo, Luana; Anastasi, Gabriele; Martinetti, Daniela; Salomone, Edvige; Colarossi, Cristina; Amato, Gabriella; Gorgone, Ausilia; Romano, Alessandra; Iannolo, Gioacchin; De Maria, Ruggero; Giustolisi, Rosario; Gulisano, Massimo; Di Raimondo, Francesco.

In: Leukemia Research, Vol. 35, No. 1, 01.2011, p. 52-60.

Research output: Contribution to journalArticle

Conticello, C, Giuffrida, R, Adamo, L, Anastasi, G, Martinetti, D, Salomone, E, Colarossi, C, Amato, G, Gorgone, A, Romano, A, Iannolo, G, De Maria, R, Giustolisi, R, Gulisano, M & Di Raimondo, F 2011, 'NF-κB localization in multiple myeloma plasma cells and mesenchymal cells', Leukemia Research, vol. 35, no. 1, pp. 52-60. https://doi.org/10.1016/j.leukres.2010.06.023
Conticello C, Giuffrida R, Adamo L, Anastasi G, Martinetti D, Salomone E et al. NF-κB localization in multiple myeloma plasma cells and mesenchymal cells. Leukemia Research. 2011 Jan;35(1):52-60. https://doi.org/10.1016/j.leukres.2010.06.023
Conticello, Concetta ; Giuffrida, Raffaella ; Adamo, Luana ; Anastasi, Gabriele ; Martinetti, Daniela ; Salomone, Edvige ; Colarossi, Cristina ; Amato, Gabriella ; Gorgone, Ausilia ; Romano, Alessandra ; Iannolo, Gioacchin ; De Maria, Ruggero ; Giustolisi, Rosario ; Gulisano, Massimo ; Di Raimondo, Francesco. / NF-κB localization in multiple myeloma plasma cells and mesenchymal cells. In: Leukemia Research. 2011 ; Vol. 35, No. 1. pp. 52-60.
@article{1c6b15cb18704f1aba03a6ff240d7111,
title = "NF-κB localization in multiple myeloma plasma cells and mesenchymal cells",
abstract = "Several reports demonstrated that the activation of Nuclear Factor-kappa B NF-κB is essential for the pathogenesis of multiple myeloma (MM). We analyzed the nuclear localization of NF-κB in MM-cells derived from 60 different patients with MM at presentation and in relapse, as well as in three myeloma cell lines. Nuclear localization (the active form) of NF-κB was detected in only one MM-sample from a refractory patient and in two samples from relapsed patients, while all the other samples, including the MM-cell lines, almost exclusively express the cytoplasmic (inactive) form of NF-κB. In mesenchymal cells from MM-patients NF-κB was clearly present in the nucleus. In addition, the proteasome inhibitor Bortezomib, which is described to antagonize NF-κB activity, had a consistent antitumor activity against both chemoresistant and chemosensitive MM-cells, regardless the NF-κB localization, thus suggesting the existence of other molecular targets of proteasome inhibitors in MM.",
keywords = "Bortezomib, Mesenchymal cells, Multiple myeloma, NF-κB",
author = "Concetta Conticello and Raffaella Giuffrida and Luana Adamo and Gabriele Anastasi and Daniela Martinetti and Edvige Salomone and Cristina Colarossi and Gabriella Amato and Ausilia Gorgone and Alessandra Romano and Gioacchin Iannolo and {De Maria}, Ruggero and Rosario Giustolisi and Massimo Gulisano and {Di Raimondo}, Francesco",
year = "2011",
month = "1",
doi = "10.1016/j.leukres.2010.06.023",
language = "English",
volume = "35",
pages = "52--60",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Ltd",
number = "1",

}

TY - JOUR

T1 - NF-κB localization in multiple myeloma plasma cells and mesenchymal cells

AU - Conticello, Concetta

AU - Giuffrida, Raffaella

AU - Adamo, Luana

AU - Anastasi, Gabriele

AU - Martinetti, Daniela

AU - Salomone, Edvige

AU - Colarossi, Cristina

AU - Amato, Gabriella

AU - Gorgone, Ausilia

AU - Romano, Alessandra

AU - Iannolo, Gioacchin

AU - De Maria, Ruggero

AU - Giustolisi, Rosario

AU - Gulisano, Massimo

AU - Di Raimondo, Francesco

PY - 2011/1

Y1 - 2011/1

N2 - Several reports demonstrated that the activation of Nuclear Factor-kappa B NF-κB is essential for the pathogenesis of multiple myeloma (MM). We analyzed the nuclear localization of NF-κB in MM-cells derived from 60 different patients with MM at presentation and in relapse, as well as in three myeloma cell lines. Nuclear localization (the active form) of NF-κB was detected in only one MM-sample from a refractory patient and in two samples from relapsed patients, while all the other samples, including the MM-cell lines, almost exclusively express the cytoplasmic (inactive) form of NF-κB. In mesenchymal cells from MM-patients NF-κB was clearly present in the nucleus. In addition, the proteasome inhibitor Bortezomib, which is described to antagonize NF-κB activity, had a consistent antitumor activity against both chemoresistant and chemosensitive MM-cells, regardless the NF-κB localization, thus suggesting the existence of other molecular targets of proteasome inhibitors in MM.

AB - Several reports demonstrated that the activation of Nuclear Factor-kappa B NF-κB is essential for the pathogenesis of multiple myeloma (MM). We analyzed the nuclear localization of NF-κB in MM-cells derived from 60 different patients with MM at presentation and in relapse, as well as in three myeloma cell lines. Nuclear localization (the active form) of NF-κB was detected in only one MM-sample from a refractory patient and in two samples from relapsed patients, while all the other samples, including the MM-cell lines, almost exclusively express the cytoplasmic (inactive) form of NF-κB. In mesenchymal cells from MM-patients NF-κB was clearly present in the nucleus. In addition, the proteasome inhibitor Bortezomib, which is described to antagonize NF-κB activity, had a consistent antitumor activity against both chemoresistant and chemosensitive MM-cells, regardless the NF-κB localization, thus suggesting the existence of other molecular targets of proteasome inhibitors in MM.

KW - Bortezomib

KW - Mesenchymal cells

KW - Multiple myeloma

KW - NF-κB

UR - http://www.scopus.com/inward/record.url?scp=78650176111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650176111&partnerID=8YFLogxK

U2 - 10.1016/j.leukres.2010.06.023

DO - 10.1016/j.leukres.2010.06.023

M3 - Article

VL - 35

SP - 52

EP - 60

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 1

ER -