NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells

Luisa Guerrini, Laura Casalino, Angelo Corti, Francesco Blasi

Research output: Contribution to journal › Article

24 Citations (Scopus)

Abstract

Binding sites for different transcription factors have been identified in the regulatory region of the human uPA gene. We investigated the role of NF-κB and AP-1 families of transcription factors in the induction of uPA mRNA by TNF-α and PMA in the A549 cell line constitutively expressing uPA mRNA and protein. Using the protein synthesis inhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF-κB and AP-1 activation, we showed that uPA mRNA induction by TNF-α and PMA in the A549 cell line is mainly due to NF-κB activation.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalFEBS Letters
Volume393
Issue number1
DOIs
Publication statusPublished - Sep 9 1996

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Urokinase-Type Plasminogen Activator
Gene Expression Regulation
Gene expression
Transcription Factor AP-1
Messenger RNA
Transcription Factors
Chemical activation
Cells
Protein Synthesis Inhibitors
Nucleic Acid Regulatory Sequences
Cycloheximide
Antioxidants
Genes
Binding Sites
A549 Cells
Proteins

Keywords

  • HIV
  • NF-κB
  • TNF-
  • uPA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells. / Guerrini, Luisa; Casalino, Laura; Corti, Angelo; Blasi, Francesco.

In: FEBS Letters, Vol. 393, No. 1, 09.09.1996, p. 69-73.

Research output: Contribution to journal › Article

Guerrini, L, Casalino, L, Corti, A & Blasi, F 1996, 'NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells', FEBS Letters, vol. 393, no. 1, pp. 69-73. https://doi.org/10.1016/0014-5793(96)00854-X
Guerrini L, Casalino L, Corti A, Blasi F. NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells. FEBS Letters. 1996 Sep 9;393(1):69-73. https://doi.org/10.1016/0014-5793(96)00854-X
Guerrini, Luisa ; Casalino, Laura ; Corti, Angelo ; Blasi, Francesco. / NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells. In: FEBS Letters. 1996 ; Vol. 393, No. 1. pp. 69-73.
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