NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells

Luisa Guerrini, Laura Casalino, Angelo Corti, Francesco Blasi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Binding sites for different transcription factors have been identified in the regulatory region of the human uPA gene. We investigated the role of NF-κB and AP-1 families of transcription factors in the induction of uPA mRNA by TNF-α and PMA in the A549 cell line constitutively expressing uPA mRNA and protein. Using the protein synthesis inhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF-κB and AP-1 activation, we showed that uPA mRNA induction by TNF-α and PMA in the A549 cell line is mainly due to NF-κB activation.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalFEBS Letters
Volume393
Issue number1
DOIs
Publication statusPublished - Sep 9 1996

Fingerprint

Urokinase-Type Plasminogen Activator
Gene Expression Regulation
Gene expression
Transcription Factor AP-1
Messenger RNA
Transcription Factors
Chemical activation
Cells
Protein Synthesis Inhibitors
Nucleic Acid Regulatory Sequences
Cycloheximide
Antioxidants
Genes
Binding Sites
A549 Cells
Proteins

Keywords

  • HIV
  • NF-κB
  • TNF-
  • uPA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells. / Guerrini, Luisa; Casalino, Laura; Corti, Angelo; Blasi, Francesco.

In: FEBS Letters, Vol. 393, No. 1, 09.09.1996, p. 69-73.

Research output: Contribution to journalArticle

Guerrini, Luisa ; Casalino, Laura ; Corti, Angelo ; Blasi, Francesco. / NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells. In: FEBS Letters. 1996 ; Vol. 393, No. 1. pp. 69-73.
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