NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells

Luisa Guerrini, Laura Casalino, Angelo Corti, Francesco Blasi

Research output: Contribution to journalArticle

Abstract

Binding sites for different transcription factors have been identified in the regulatory region of the human uPA gene. We investigated the role of NF-κB and AP-1 families of transcription factors in the induction of uPA mRNA by TNF-α and PMA in the A549 cell line constitutively expressing uPA mRNA and protein. Using the protein synthesis inhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF-κB and AP-1 activation, we showed that uPA mRNA induction by TNF-α and PMA in the A549 cell line is mainly due to NF-κB activation.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalFEBS Letters
Volume393
Issue number1
DOIs
Publication statusPublished - Sep 9 1996

Keywords

  • HIV
  • NF-κB
  • TNF-
  • uPA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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