Abstract
Binding sites for different transcription factors have been identified in the regulatory region of the human uPA gene. We investigated the role of NF-κB and AP-1 families of transcription factors in the induction of uPA mRNA by TNF-α and PMA in the A549 cell line constitutively expressing uPA mRNA and protein. Using the protein synthesis inhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF-κB and AP-1 activation, we showed that uPA mRNA induction by TNF-α and PMA in the A549 cell line is mainly due to NF-κB activation.
| Original language | English |
|---|---|
| Pages (from-to) | 69-73 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 393 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sep 9 1996 |
Keywords
- HIV
- NF-κB
- TNF-
- uPA
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Guerrini, L., Casalino, L., Corti, A., & Blasi, F. (1996). NF-κB-mediated regulation of urokinase gene expression by PMA and TNF-α in human A549 cells. FEBS Letters, 393(1), 69-73. https://doi.org/10.1016/0014-5793(96)00854-X