NF-κB oscillations translate into functionally related patterns of gene expression

Samuel Zambrano, Ilario de Toma, Arianna Piffer, Marco E. Bianchi, Alessandra Agresti

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Several transcription factors (TFs) oscillate, periodically relocating between the cytoplasm and the nucleus. NF-KB, which plays key roles in inflammation and cancer, displays oscillations whose biological advantage remains unclear. Recent work indicated that NF-KB displays sustained oscillations that can be entrained, that is, reach a persistent synchronized state through small periodic perturbations. We show here that for our GFP-p65 knock-in cells NF-KB behaves as a damped oscillator able to synchronize to a variety of periodic external perturbations with no memory. We imposed synchronous dynamics to prove that transcription of NF-KB-controlled genes also oscillates, but mature transcript levels follow three distinct patterns. Two sets of transcripts accumulate fast or slowly, respectively. Another set, comprising chemokine and chemokine receptor mRNAs, oscillates and resets at each new stimulus, with no memory of the past. We propose that TF oscillatory dynamics is a means of segmenting time to provide renewing opportunity windows for decision.

Original languageEnglish
Article numbere09100
JournaleLife
Volume5
Issue numberJANUARY2016
DOIs
Publication statusPublished - Jan 14 2016

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Gene expression
Transcription Factors
KB Cells
Gene Expression
Data storage equipment
Chemokine Receptors
Transcription
Chemokines
Cytoplasm
Genes
Display devices
Inflammation
Messenger RNA
Neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

NF-κB oscillations translate into functionally related patterns of gene expression. / Zambrano, Samuel; de Toma, Ilario; Piffer, Arianna; Bianchi, Marco E.; Agresti, Alessandra.

In: eLife, Vol. 5, No. JANUARY2016, e09100, 14.01.2016.

Research output: Contribution to journalArticle

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