NG2, a common denominator for neuroinflammation, blood–brain barrier alteration, and oligodendrocyte precursor response in EAE, plays a role in dendritic cell activation

Giovanni Ferrara, Mariella Errede, Francesco Girolamo, Sara Morando, Federico Ivaldi, Nicolò Panini, Caterina Bendotti, Roberto Perris, Roberto Furlan, Daniela Virgintino, Nicole Kerlero de Rosbo, Antonio Uccelli

Research output: Contribution to journalArticle

Abstract

In adult CNS, nerve/glial-antigen 2 (NG2) is expressed by oligodendrocyte progenitor cells (OPCs) and is an early marker of pericyte activation in pathological conditions. NG2 could, therefore, play a role in experimental autoimmune encephalomyelitis (EAE), a disease associated with increased blood–brain barrier (BBB) permeability, inflammatory infiltrates, and CNS damage. We induced EAE in NG2 knock-out (NG2KO) mice and used laser confocal microscopy immunofluorescence and morphometry to dissect the effect of NG2 KO on CNS pathology. NG2KO mice developed milder EAE than their wild-type (WT) counterparts, with less intense neuropathology associated with a significant improvement in BBB stability. In contrast to WT mice, OPC numbers did not change in NG2KO mice during EAE. Through FACS and confocal microscopy, we found that NG2 was also expressed by immune cells, including T cells, macrophages, and dendritic cells (DCs). Assessment of recall T cell responses to the encephalitogen by proliferation assays and ELISA showed that, while WT and NG2KO T cells proliferated equally to the encephalitogenic peptide MOG35-55, NG2KO T cells were skewed towards a Th2-type response. Because DCs could be responsible for this effect, we assessed their expression of IL-12 by PCR and intracellular FACS. IL-12-expressing CD11c+ cells were significantly decreased in MOG35-55-primed NG2KO lymph node cells. Importantly, in WT mice, the proportion of IL-12-expressing cells was significantly lower in CD11c+ NG2- cells than in CD11c+ NG2+ cells. To assess the relevance of NG2 at immune system and CNS levels, we induced EAE in bone-marrow chimeric mice, generated with WT recipients of NG2KO bone-marrow cells and vice versa. Regardless of their original phenotype, mice receiving NG2KO bone marrow developed milder EAE than those receiving WT bone marrow. Our data suggest that NG2 plays a role in EAE not only at CNS/BBB level, but also at immune response level, impacting on DC activation and thereby their stimulation of reactive T cells, through controlling IL-12 expression.
Original languageEnglish
Pages (from-to)23 - 42
Number of pages20
JournalActa Neuropathologica
Volume132
Issue number1
DOIs
Publication statusPublished - Jul 1 2016

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Autoimmune Experimental Encephalomyelitis
Oligodendroglia
Neuroglia
Dendritic Cells
Antigens
Interleukin-12
T-Lymphocytes
Knockout Mice
Confocal Microscopy
Bone Marrow
Stem Cells
Pericytes
Bone Marrow Cells
Fluorescent Antibody Technique
Immune System
Permeability
Cell Count
Lymph Nodes
Enzyme-Linked Immunosorbent Assay
Macrophages

Keywords

  • Blood–brain barrier
  • DC activation
  • Experimental autoimmune encephalomyelitis
  • Nerve/glial-antigen 2
  • Oligodendrocyte precursor cells
  • Tight junctions

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

NG2, a common denominator for neuroinflammation, blood–brain barrier alteration, and oligodendrocyte precursor response in EAE, plays a role in dendritic cell activation. / Ferrara, Giovanni; Errede, Mariella; Girolamo, Francesco; Morando, Sara; Ivaldi, Federico; Panini, Nicolò; Bendotti, Caterina; Perris, Roberto; Furlan, Roberto; Virgintino, Daniela; Kerlero de Rosbo, Nicole; Uccelli, Antonio.

In: Acta Neuropathologica, Vol. 132, No. 1, 01.07.2016, p. 23 - 42.

Research output: Contribution to journalArticle

Ferrara, Giovanni ; Errede, Mariella ; Girolamo, Francesco ; Morando, Sara ; Ivaldi, Federico ; Panini, Nicolò ; Bendotti, Caterina ; Perris, Roberto ; Furlan, Roberto ; Virgintino, Daniela ; Kerlero de Rosbo, Nicole ; Uccelli, Antonio. / NG2, a common denominator for neuroinflammation, blood–brain barrier alteration, and oligodendrocyte precursor response in EAE, plays a role in dendritic cell activation. In: Acta Neuropathologica. 2016 ; Vol. 132, No. 1. pp. 23 - 42.
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AU - Morando, Sara

AU - Ivaldi, Federico

AU - Panini, Nicolò

AU - Bendotti, Caterina

AU - Perris, Roberto

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